Journal of Experimental Botany, Vol. 54, No. 381, pp. 279-290,
January 2, 2003
© 2003 Oxford University Press
Isolation of GUS marker lines for genes expressed in Arabidopsis endosperm, embryo and maternal tissues
Received 19 June 2002; Accepted 11 September 2002
1 Plant Molecular Biology Laboratory, Department of Chemistry and Biotechnology, Agricultural University of Norway, PO Box 5040, N-1432 Aas, Norway
2 Division of Molecular Biology, University of Oslo, Oslo, Norway
3 University of Skövde, Sweden
4 Present address: Pioneer Hi-Bred International, A DuPont Company, Iowa 7300 NW 62nd Avenue, Johnston, IA 50131–1004, USA.
5 To whom correspondence should be addressed. Fax: +47 64 94 77 20. E-mail: Biljana.Stangeland{at}ikb.nlh.no
In order to identify marker lines expressing GUS in various endosperm compartments and at different developmental stages, a collection of Arabidopsis thaliana (L.) Heynh. promoter trap lines were screened. The screen identified 16 lines displaying GUS-reporter gene expression in the endosperm, embryo and other seed organs. The distinctive patterns of GUS expression in these lines provide molecular markers for most cell compartments in the endosperm of Arabidopsis seeds at all developmental stages, and represent a valuable research tool for characterizing present and future Arabidopsis seed mutants. GUS expression patterns of these 16 lines are presented here. One line showed chalazal endosperm-specific GUS activity at the heart stage of embryo development. In six lines embryo-specific GUS activity was detected. Six lines exhibited GUS activity predominantly in the endosperm and embryo while two lines showed strong GUS activity in all seed organs. In one line GUS activity was detected in integuments and syncytial endosperm, while the GUS activity at the cotyledonary stage of the embryo was seed coat-specific. In addition, two funiculus markers and two silique markers expressed in the abscission zone and the guard cells are also presented.
Key words: Arabidopsis, clearing, embryo, endosperm, GUS, seed, promoter, trapping.
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