Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Freeman, D.
Right arrow Articles by Murray, J. A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Freeman, D.
Right arrow Articles by Murray, J. A. H.
Agricola
Right arrow Articles by Freeman, D.
Right arrow Articles by Murray, J. A. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 381, pp. 303-308, January 2, 2003
© 2003 Oxford University Press

Isolation, characterization and expression of cyclin and cyclin-dependent kinase genes in Jerusalem artichoke (Helianthus tuberosus L.)

Received 10 February 2002; Accepted 18 September 2002

Donna Freeman1, Catherine Riou-Khamlichi2, E. Ann Oakenfull and James A. H. Murray3

Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK

1 Present address: Acambis plc, Peterhouse Technology Park, 100 Fulbourn Road, Cambridge CB1 9PT, UK.
2 Present address: Institut des Sciences de la Vie et de la Santé, Faculté des Sciences, Université de Limoges, 87100 Limoges, France.
3 To whom correspondence should be addressed. Fax: +44 (0)1223 334162. E-mail: j.murray{at}biotech.cam.ac.uk
Abbreviations: CDK, cyclin-dependent kinase; ABA, abscisic acid; ORF, open reading frame; RT-PCR, reverse-transcriptase PCR.

Tuber explants of Jerusalem artichoke (Helianthus tuberosus L.) are a model system for cell-cycle re-entry from a quiescent state, involving the activation of division of tuber parenchyma cells in response to exogenous auxin. To enable molecular studies of this system, two cyclin (Heltu;CYCD1;1 and Heltu; CYCD3;1) and two cyclin-dependent kinase (Heltu; CDKA;1 and Heltu;CDKB1;1) genes have been isolated from a Jerusalem artichoke cDNA library and their expression demonstrated during the activation of cell division. It was found that CDKA;1 transcripts are present in quiescent tubers, whereas CYCD1;1, CYCD3;1 and CDKB1;1 transcripts are induced during cell-cycle re-entry as well as during bud growth of whole tubers. Both CYCD1;1 and CYCD3;1 transcripts appear shortly before, or coincident with, the onset of S phase.

Key words: Cyclin, cyclin-dependent kinase, Helianthus tuberosus.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
O. A. Koroleva, M. Tomlinson, P. Parinyapong, L. Sakvarelidze, D. Leader, P. Shaw, and J. H. Doonan
CycD1, a Putative G1 Cyclin from Antirrhinum majus, Accelerates the Cell Cycle in Cultured Tobacco BY-2 Cells by Enhancing Both G1/S Entry and Progression through S and G2 Phases
PLANT CELL, September 1, 2004; 16(9): 2364 - 2379.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.