Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Dambrauskas, G.
Right arrow Articles by Rogers, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dambrauskas, G.
Right arrow Articles by Rogers, H. J.
Agricola
Right arrow Articles by Dambrauskas, G.
Right arrow Articles by Rogers, H. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 383, pp. 699-706, February 1, 2003
© 2003 Oxford University Press

Genes encoding two essential DNA replication activation proteins, Cdc6 and Mcm3, exhibit very different patterns of expression in the tobacco BY-2 cell cycle

Received 4 July 2002; Accepted 14 October 2002

Gerardas Dambrauskas4,1, Stephen J. Aves1, John A. Bryant3,1, Dennis Francis2 and Hilary J. Rogers2

1 School of Biological Sciences, Washington Singer Laboratories, University of Exeter, Exeter EX4 4QG, UK
2 School of Biosciences, Cardiff University, PO Box 915, Cardiff CF10 3TL, UK

3 To whom correspondence should be addressed. Fax: +44 (0)1392 264668. E-mail: J.A.Bryant{at}exeter.ac.uk
4 Present address: Department of Biochemistry, North Carolina State University, Raleigh, NC 27695, USA.

Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as probes in northern blots which suggested that, in the cell cycle of synchronized tobacco BY-2 cells, expression of CDC6 is confined to late G1 and S-phase whereas the expression of MCM3 is not confined to any particular cell cycle phase. These data were confirmed and extended by real-time PCR measurements of mRNA abundance through the cell cycle. CDC6 exhibits a very clear peak of expression in S-phase whereas MCM3, expressed at a much lower level than CDC6, is not cell-cycle-regulated. These patterns of cell cycle gene expression resemble those found in the fission yeast Schizosaccharomyces pombe rather than those in budding yeast or mammalian cells.

Key words: BY-2, Cdc6, cell cycle, DNA replication, Mcm3, tobacco.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
D. Francis, M. S. Davies, and P. W. Barlow
A Strong Nucleotypic Effect on the Cell Cycle Regardless of Ploidy Level
Ann. Bot., April 1, 2008; 101(6): 747 - 757.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. W. Shultz, V. M. Tatineni, L. Hanley-Bowdoin, and W. F. Thompson
Genome-Wide Analysis of the Core DNA Replication Machinery in the Higher Plants Arabidopsis and Rice
Plant Physiology, August 1, 2007; 144(4): 1697 - 1714.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Gachon, A. Mingam, and B. Charrier
Real-time PCR: what relevance to plant studies?
J. Exp. Bot., July 1, 2004; 55(402): 1445 - 1454.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.