Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Meyer, S.
Right arrow Articles by Cerovic, Z. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meyer, S.
Right arrow Articles by Cerovic, Z. G.
Agricola
Right arrow Articles by Meyer, S.
Right arrow Articles by Cerovic, Z. G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 383, pp. 757-769, February 1, 2003
© 2003 Oxford University Press

UV-induced blue-green and far-red fluorescence along wheat leaves: a potential signature of leaf ageing

Received 16 September 2002; Accepted 3 October 2002

S. Meyer1,, A. Cartelat, I. Moya and Z. G. Cerovic

Equipe Photosynthèse et Télédétection, LURE/CNRS, Bât 203, Centre Universitaire Paris-Sud, BP34, F-91898 Orsay Cedex, France

1 To whom correspondence should be sent: Fax: 33 1 64 46 41 48. E-mail: sylvie.meyer{at}lure.u-psud.fr

Under UV-excitation, leaves emit red (RF) and far-red (FRF) fluorescence from chlorophyll and blue-green fluorescence (BGF) from hydroxycinnamic acids. In this study, the aim was to develop a fluorescence signature of wheat leaf ageing after the emergence of the lamina. FRF and BGF were examined in the first three leaves of 2-week-old wheat plants. It was investigated how FRF and BGF vary as leaf and tissue aged by spectroscopic measurements, time-resolved BGF analysis and microscopic imaging of the leaf surface. It was found that FRF decreased with leaf and tissue ageing because of an accumulation of UV-absorbers in the epidermis. BGF also decreased, but without changes either in the shape of excitation and emission spectra or in the fluorescence lifetime. So, BGF emanated from the leaf surface, without changes in fluorophore composition during leaf ageing. The shape of the BGF spectrum indicates that ferulic acid bound to the cell wall is the main blue-green fluorophore. The effects of pH and solvents on BGF from intact leaves and ferulic acid in solution were similar, confirming the hydroxycinnamic acid origin of BGF. UV-fluorescence microscopic imaging of the surface of intact leaves showed that different epidermis cell types and sclerenchyma bands emitted BGF. The decreasing gradient of BGF from the base to the apex of the lamina could be related to the decrease in the surface of the fluorescent sclerenchyma bands. The significance of FRF and BGF as potential signatures of wheat lamina growth are discussed.

Key words: Blue-green fluorescence, ferulic acid, fluorescence microscopic imaging, leaf fluorescence, Triticum aestivum L.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
S. Meyer, J. Louis, N. Moise, T. Piolot, X. Baudin, and Z. G. Cerovic
Developmental changes in spatial distribution of in vivo fluorescence and epidermal UV absorbance over Quercus petraea leaves
Ann. Bot., September 1, 2009; 104(4): 621 - 633.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. Malenovsky, K. B. Mishra, F. Zemek, U. Rascher, and L. Nedbal
Scientific and technical challenges in remote sensing of plant canopy reflectance and fluorescence
J. Exp. Bot., July 1, 2009; 60(11): 2987 - 3004.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. Solti, L. Gaspar, I. Meszaros, Z. Szigeti, L. Levai, and E. Sarvari
Impact of Iron Supply on the Kinetics of Recovery of Photosynthesis in Cd-stressed Poplar (Populus glauca)
Ann. Bot., November 1, 2008; 102(5): 771 - 782.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
T. Hura, S. Grzesiak, K. Hura, E. Thiemt, K. Tokarz, and M. Wedzony
Physiological and Biochemical Tools Useful in Drought-Tolerance Detection in Genotypes of Winter Triticale: Accumulation of Ferulic Acid Correlates with Drought Tolerance
Ann. Bot., October 1, 2007; 100(4): 767 - 775.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.