Journal of Experimental Botany, Vol. 54, No. 383, pp. 801-812,
February 1, 2003
© 2003 Oxford University Press
Mapping of QTL associated with nitrogen storage and remobilization in barley (Hordeum vulgare L.) leaves
Received 19 June 2002; Accepted 22 October 2002
1 Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717-3150, USA
2 Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
3 International Center for Agricultural Research in the Dry Areas, PO Box 5466, Aleppo, Syrian Arab Republic
4 To whom correspondence should be addressed. Fax: +1 406 994 7600. E-mail: fischer{at}montana.edu
Nitrogen uptake and metabolism are central for vegetative and reproductive plant growth. This is reflected by the fact that nitrogen can be remobilized and reused within a plant, and this process is crucial for yield in most annual crops. A population of 146 recombinant inbred barley lines (F8 and F9 plants, grown in 2000 and 2001), derived from a cross between two varieties differing markedly in grain protein concentration, was used to compare the location of QTL associated with nitrogen uptake, storage and remobilization in flag leaves relative to QTL controlling developmental parameters and grain protein accumulation. Overlaps of support intervals for such QTL were found on several chromosomes, with chromosomes 3 and 6 being especially important. For QTL on these chromosomes, alleles associated with inefficient N remobilization were associated with depressed yield and higher levels of total or soluble organic nitrogen during grain filling and vice versa; therefore, genes directly involved in N recycling or genes regulating N recycling may be located on these chromosomes. Interestingly, the most prominent QTL for grain protein concentration (on chromosome 6) did not co-localize with QTL for nitrogen remobilization. However, QTL peaks for nitrate and soluble organic nitrogen were detected at this locus for plants grown in 2001 (but not in 2000). For these, alleles associated with low grain protein concentration were associated with higher soluble nitrogen levels in leaves during grain filling; therefore, gene(s) found at this locus might influence the nitrogen sink strength of developing barley grains.
Key words: Barley, grain protein concentration, Hordeum vulgare L., nitrogen remobilization, nitrogen storage, QTL, yield.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S.-C. Fan, C.-S. Lin, P.-K. Hsu, S.-H. Lin, and Y.-F. Tsay The Arabidopsis Nitrate Transporter NRT1.7, Expressed in Phloem, Is Responsible for Source-to-Sink Remobilization of Nitrate PLANT CELL, September 1, 2009; 21(9): 2750 - 2761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. C. Collins, F. Tardieu, and R. Tuberosa Quantitative Trait Loci and Crop Performance under Abiotic Stress: Where Do We Stand? Plant Physiology, June 1, 2008; 147(2): 469 - 486. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tabuchi, T. Abiko, and T. Yamaya Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.) J. Exp. Bot., July 1, 2007; 58(9): 2319 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hirel, J. Le Gouis, B. Ney, and A. Gallais The challenge of improving nitrogen use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches J. Exp. Bot., July 1, 2007; 58(9): 2369 - 2387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fan, L. Jia, Y. Li, S. J. Smith, A. J. Miller, and Q. Shen Comparing nitrate storage and remobilization in two rice cultivars that differ in their nitrogen use efficiency J. Exp. Bot., May 1, 2007; 58(7): 1729 - 1740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Carollo, D. E. Matthews, G. R. Lazo, T. K. Blake, D. D. Hummel, N. Lui, D. L. Hane, and O. D. Anderson GrainGenes 2.0. An Improved Resource for the Small-Grains Community Plant Physiology, October 1, 2005; 139(2): 643 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. MALAGOLI, P. LAINE, L. ROSSATO, and A. OURRY Dynamics of Nitrogen Uptake and Mobilization in Field-grown Winter Oilseed Rape (Brassica napus) From Stem Extension to Harvest. II. An 15N-labelling-based Simulation Model of N Partitioning Between Vegetative and Reproductive Tissues Ann. Bot., June 1, 2005; 95(7): 1187 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yang, S. Mickelson, D. See, T. K. Blake, and A. M. Fischer Genetic analysis of the function of major leaf proteases in barley (Hordeum vulgare L.) nitrogen remobilization J. Exp. Bot., December 1, 2004; 55(408): 2607 - 2616. [Abstract] [Full Text] [PDF]
|
|