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Journal of Experimental Botany, Vol. 54, No. 384, pp. 1117-1119, March 1, 2003
© 2003 Oxford University Press

Zea mays ZmMybst1 cDNA, encodes a single Myb-repeat protein with the VASHAQKYF motif

Received 2 December 2002; Accepted 17 December 2002

Inderjit Singh Mercy4,2, Robert B. Meeley3, Scott E. Nichols3 and Odd-Arne Olsen2

2 PO Box 5051, Agricultural University of Norway, 1432 Aas, Norway
3 Pioneer Hi-Bred International, PO Box 1004, Johnston, 1A 50131-1004, USA

1 The gene sequence has been submitted by Bankit: the identification number is 513311.
4 To whom correspondence should be addressed. E-mail: Inderjit.mercy{at}bio.uio.no

A cDNA clone from a 4 DAP dissected maize embryo sac encoding a novel Zea mays single-repeat Myb protein is reported here. This full-length cDNA contains an ORF of 948 bp. The gene ZmMybst1 contains two introns (1166 and 706 bp) and is a single copy gene. The ZmMybst1 protein shares high sequence identity with the potato Mybst1 protein (58%). Northern blot, RT-PCR and electronic northern analysis shows that ZmMybst1 is expressed in endosperm between 4 and 30 DAP, coinciding with the period of aleurone cell differentiation and development.

Key words: expression analysis, Myb, ZmMybst1.


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