Journal of Experimental Botany, Vol. 54, No. 384, pp. 951-959,
March 1, 2003
© 2003 Oxford University Press
A cathepsin B-like cysteine protease gene from Hordeum vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves
Received 8 August 2002; Accepted 4 November 2002
Laboratorio de Bioquímica y Biología Molecular, Dpto. de Biotecnología, ETSI Agrónomos-UPM, Ciudad Universitaria s/n, 28040 Madrid, Spain
1 The nucleotide sequence data reported will appear in the EMBL Nucleotide Sequence Database under accession number AJ310426.
2 To whom correspondence should be sent. Fax:+34 913365757. e-mail: isabel{at}bit.etsia.upm.es
Abbreviations: GA, gibberellin; ABA, abscisic acid.
A barley cDNA clone encoding a putative cysteine protease with sequence homology to cathepsin B from mammalian cells has been characterized. This barley gene (CatB) is ubiquitously expressed, its mRNA being detected in leaves and roots, immature, mature and germinating embryos, in developing endosperms, and in aleurones upon germination, as assessed by northern blot analysis. The CatB mRNA expression in leaves increased by cold shock (6 °C), was not affected by wounding, and was under circadian control. These transcripts increased in the aleurone upon germination, whereas those for a cystatin encoding gene (Icy), that inhibits commercial cathepsin B in vitro, decreased. Gibberellin (GA) treatment of isolated barley aleurones induced and abscisic acid (ABA) repressed the steady-state levels of CatB mRNA, while Icy expression had an opposite pattern of mRNA accumulation in aleurones treated with GA. No response to GA or ABA was detected in leaves.
Key words: Abiotic stress, barley cathepsin-B expression, cDNA cloning, circadian rhythm, germination hormonal regulation.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Abraham, M. Martinez, P. Carbonero, and I. Diaz Structural and functional diversity within the cystatin gene family of Hordeum vulgare J. Exp. Bot., December 1, 2006; 57(15): 4245 - 4255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Jang, T. L. Kamps, D. N. Skinner, S. R. Schulze, W. K. Vencill, and A. H. Paterson Functional Classification, Genomic Organization, Putatively cis-Acting Regulatory Elements, and Relationship to Quantitative Trait Loci, of Sorghum Genes with Rhizome-Enriched Expression Plant Physiology, November 1, 2006; 142(3): 1148 - 1159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Martinez, I. Rubio-Somoza, R. Fuentes, P. Lara, P. Carbonero, and I. Diaz The barley cystatin gene (Icy) is regulated by DOF transcription factors in aleurone cells upon germination J. Exp. Bot., February 1, 2005; 56(412): 547 - 556. [Abstract] [Full Text] [PDF]
|
|