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JXB Advance Access originally published online on March 3, 2003
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Journal of Experimental Botany, Vol. 54, No. 385, pp. 1153-1162, April 1, 2003
© 2003 Oxford University Press

A jasmonate-responsive element within the A. thaliana vsp1 promoter

Received 13 September 2002; Accepted 2 January 2003

F. Guerineau4,1, M. Benjdia2 and D. X. Zhou3

1 Université de Picardie Jules Verne, 33 rue St Leu, F-80039 Amiens, France
2 ZMBP, Plant Physiology, Auf der Morgenstelle 1, Universität Tubingen, D-72076, Tubingen, Germany
3 Institut de biotechnologie des plantes, Université de Paris-sud, F-91405 Orsay, France

4 To whom correspondence should be addressed. Fax: +33 3 22 82 79 76. E-mail: francois.guerineau{at}sc.u-picardie.fr

The vsp1 gene of Arabidopsis thaliana encodes a storage protein that accumulates in vegetative organs. Transgenic plants expressing a vsp1 promoter–gus (ß-glucuronidase) gene fusion were found to contain high GUS activity when challenged with jasmonate, a volatile plant hormone. The induction of vsp1–gus expression by jasmonate could be measured in tobacco mesophyll protoplasts, after transient expression. A number of deletions were operated in the vsp1 promoter in order to locate its jasmonate-responsive element. A 41 bp sequence taken approximately 150 bp upstream of the vsp1 TATA box could confer jasmonate responsiveness upon a short CaMV 35S promoter. Whereas the deletion of a CAAT box-like element within the 41 bp sequence did not affect promoter activity, mutation of a short palindromic motif completely abolished jasmonate responsiveness. This motif shows no homology with the jasmonate-responsive elements of other promoters.

Key words: Arabidopsis thaliana, gene expression, jasmonate, promoter, vegetative storage protein.


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