Journal of Experimental Botany, Vol. 54, No. 385, pp. 1163-1174,
April 1, 2003
© 2003 Oxford University Press
Aluminium-responsive genes in sugarcane: identification and analysis of expression under oxidative stress
Received 19 July 2002; Accepted 10 January 2003
Biotechnology Department, South African Sugar Association Experiment Station, Private Bag X02, Mount Edgecombe 4300, South Africa
1 Fax: +27 31 5395406. E-mail: watt{at}sugar.org.za
Suppression subtractive hybridization (SSH) technology was used to gain preliminary insights into gene expression induced by the phytotoxic aluminium species, Al3+, in sugarcane roots. Roots of hydroponically-grown Saccharum spp. hybrid cv. N19 were exposed to 221 µM Al3+ at pH 4.1 for 24 h, a regime shown to inhibit root elongation by 43%, relative to unchallenged roots. Database comparisons revealed that, of a subset of 50 cDNAs ostensibly up-regulated by the metal in the root tips, 14 possessed putative identities indicative of involvement in signalling events and the regulation of gene expression, while the majority (28) were of unknown function. All of the 50 cDNAs sequenced displayed significant similarity to uncharacterized plant expressed sequence tags (ESTs), approximately half (23) of which had been derived from other graminaceous crop species that had been subject to a variety of stresses. Analysis of the expression of 288 putative Al3+-inducible genic fragments indicated higher levels of expression under oxidative (1 mM diamide for 4 h) rather than Al3+ stress. By deploying SSH, this study has provided an indication of the nature of genes expressed in sugarcane roots under Al3+ stress. It is anticipated that the information obtained will guide further exploration of the potential for manipulation of the Al tolerance characteristics of the crop.
Key words: Aluminium, oxidative stress, sugarcane, suppression subtractive hybridization.
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