Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Watt, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Watt, D. A.
Agricola
Right arrow Articles by Watt, D. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 385, pp. 1163-1174, April 1, 2003
© 2003 Oxford University Press

Aluminium-responsive genes in sugarcane: identification and analysis of expression under oxidative stress

Received 19 July 2002; Accepted 10 January 2003

Derek A. Watt1,

Biotechnology Department, South African Sugar Association Experiment Station, Private Bag X02, Mount Edgecombe 4300, South Africa

1 Fax: +27 31 5395406. E-mail: watt{at}sugar.org.za

Suppression subtractive hybridization (SSH) technology was used to gain preliminary insights into gene expression induced by the phytotoxic aluminium species, Al3+, in sugarcane roots. Roots of hydroponically-grown Saccharum spp. hybrid cv. N19 were exposed to 221 µM Al3+ at pH 4.1 for 24 h, a regime shown to inhibit root elongation by 43%, relative to unchallenged roots. Database comparisons revealed that, of a subset of 50 cDNAs ostensibly up-regulated by the metal in the root tips, 14 possessed putative identities indicative of involvement in signalling events and the regulation of gene expression, while the majority (28) were of unknown function. All of the 50 cDNAs sequenced displayed significant similarity to uncharacterized plant expressed sequence tags (ESTs), approximately half (23) of which had been derived from other graminaceous crop species that had been subject to a variety of stresses. Analysis of the expression of 288 putative Al3+-inducible genic fragments indicated higher levels of expression under oxidative (1 mM diamide for 4 h) rather than Al3+ stress. By deploying SSH, this study has provided an indication of the nature of genes expressed in sugarcane roots under Al3+ stress. It is anticipated that the information obtained will guide further exploration of the potential for manipulation of the Al tolerance characteristics of the crop.

Key words: Aluminium, oxidative stress, sugarcane, suppression subtractive hybridization.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 jashsHome page
M. Reyes-Diaz, M. Alberdi, and M. d. l. L. Mora
Short-term Aluminum Stress Differentially Affects the Photochemical Efficiency of Photosystem II in Highbush Blueberry Genotypes
J. Amer. Soc. Hort. Sci., January 1, 2009; 134(1): 14 - 21.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Zhang, Z. He, H. Tian, G. Zhu, and X. Peng
Identification of aluminium-responsive genes in rice cultivars with different aluminium sensitivities
J. Exp. Bot., June 1, 2007; 58(8): 2269 - 2278.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Zhang, M. A. R. Mian, K. Chekhovskiy, S. So, D. Kupfer, H. Lai, and B. A. Roe
Differential gene expression in Festuca under heat stress conditions
J. Exp. Bot., March 1, 2005; 56(413): 897 - 907.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Mao, K. Yi, L. Yang, B. Zheng, Y. Wu, F. Liu, and P. Wu
Identification of aluminium-regulated genes by cDNA-AFLP in rice (Oryza sativa L.): aluminium-regulated genes for the metabolism of cell wall components
J. Exp. Bot., January 1, 2004; 55(394): 137 - 143.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.