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Journal of Experimental Botany, Vol. 54, No. 385, pp. 1269-1280, April 1, 2003
© 2003 Oxford University Press

Sodium chloride reduces growth and cytosolic calcium, but does not affect cytosolic pH, in root hairs of Arabidopsis thaliana L.

Received 16 September 2002; Accepted 14 January 2003

Stephen J. Halperin1, Simon Gilroy2 and Jonathan P. Lynch3,1

1 Department of Horticulture, The Pennsylvania State University, 102 Tyson Bldg., University Park, PA 16802, USA
2 Department of Biology, The Pennsylvania State University, 208 Mueller Bldg., University Park, PA 16802, USA

3 To whom correspondence should be addressed. Fax: +1 814 863 6139. E-mail: JPL4{at}psu.edu

The effects of salinity (NaCl) stress on growth, cytosolic Ca2+ gradients and cytosolic pH homeostasis of root hairs of Arabidopsis thaliana are assessed here. Neither cytosolic Ca2+ nor pH at the hair apex were significantly affected by 20 min exposure of up to 90 mM NaCl or of up to 5 mM extracellular Ca2+. Exposure to increasing NaCl concentrations, up to 90 mM, for 2 d or 6 d reduced hair extension, and this inhibition was relieved by supplemental extracellular Ca2+. Such extended salinity stress reduced the magnitude of the Ca2+ gradient in the apical 12 µm of hairs at all NaCl concentrations tested (up to 90 mM), including NaCl concentrations that did not reduce hair extension. The magnitude of the tip-focused gradient was also reduced in root hairs of plants grown with low (0.5 mM) extracellular Ca2+ when compared to those in 5 mM extracellular Ca2+, regardless of the presence of NaCl. Up to 90 mM NaCl did not affect cytosolic pH of root hairs in any of the treatments. It is concluded that NaCl inhibition of root hair extension in the long term may operate via alterations in the tip-focused Ca2+ gradient that regulates root hair growth. However, NaCl-induced alterations in this gradient do not always lead to detectably altered growth kinetics. Short-term signalling events in response to NaCl may operate by a means other than altering Ca2+ at the root hair apex. Salinity stress in root hairs does not appear to be mediated by effects on cytosolic pH.

Key words: BCECF, cytosolic calcium, cytosolic pH, Indo-1, root hair, salinity, sodium chloride.


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