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JXB Advance Access originally published online on April 11, 2003
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Journal of Experimental Botany, Vol. 54, No. 387, pp. 1577-1584, June 1, 2003
© 2003 Oxford University Press

Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins

Received 3 September 2002; Accepted 26 February 2003

Ricardo Flückiger2, Monica De Caroli1, Gabriella Piro1, Giuseppe Dalessandro1, Jean-Marc Neuhaus2 and Gian-Pietro Di Sansebastiano3,1

1 Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, via pro.le Lecce-Monteroni, I-73100 Lecce, Italy
2 Laboratoire de Biochimie, Institute de Botanique, Université de Neuchâtel, rue Emile-Argand 9, CP 2, CH-2007 Neuchâtel 7, Switzerland

3 To whom correspondence should be addressed. Fax: +39 832 320626. E-mail: gp.disansebastiano{at}unile.it

Green fluorescent protein (GFP) allows the direct visualization of gene expression and the subcellular localization of fusion proteins in living cells. The localization of different GFP fusion proteins in the secretory system was studied in stably transformed Arabidopsis plants cv. Wassilewskaja. Secreted GFP (SGFP) and GFP retained in the ER (GFP-KDEL) confirmed patterns already known, but two vacuolar GFPs (GFP-Chi and Aleu-GFP) labelled the Arabidopsis vacuolar system for the first time, the organization of which appears to depend on cell differentiation. GFP stability in the vacuoles may depend on pH or degradation, but these vacuolar markers can, nevertheless, be used as a tool for physiological studies making these plants suitable for mutagenesis and gene-tagging experiments.

Key words: Arabidopsis, development, GFP, soluble protein sorting, vacuole biogenesis.


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