JXB Advance Access originally published online on May 13, 2003
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Journal of Experimental Botany, Vol. 54, No. 388, pp. 1701-1709,
July 1, 2003
© 2003 Oxford University Press
Effect of glucose on assimilatory sulphate reduction in Arabidopsis thaliana roots
Received 13 December 2002; Accepted 24 March 2003
1 Max Planck Institute for Molecular Plant Physiology, Department of Molecular Physiology, Am Muehlenberg 1, D-14476 Golm, Germany
2 University of Bern, Institute of Plant Sciences, Altenbergrain 21, 3013 Bern, Switzerland
3 University of Freiburg, Institute of Forest Botany and Tree Physiology, Georges-Köhler-Allee, Geb. 053/054, D-79085 Freiburg, Germany
* To whom the correspondence should be addressed. Fax: +49 331 5678201. E-mail: hesse{at}mpimp-golm.mpg.de
With the aim of analysing the relative importance of sugar supply and nitrogen nutrition for the regulation of sulphate assimilation, the regulation of adenosine 5'-phosphosulphate reductase (APR), a key enzyme of sulphate reduction in plants, was studied. Glucose feeding experiments with Arabidopsis thaliana cultivated with and without a nitrogen source were performed. After a 38 h dark period, APR mRNA, protein, and enzymatic activity levels decreased dramatically in roots. The addition of 0.5% (w/v) glucose to the culture medium resulted in an increase of APR levels in roots (mRNA, protein and activity), comparable to those of plants kept under normal light conditions. Treatment of roots with D-sorbitol or D-mannitol did not increase APR activity, indicating that osmotic stress was not involved in APR regulation. The addition of O-acetyl-L-serine (OAS) also quickly and transiently increased APR levels (mRNA, protein, and activity). Feeding plants with a combination of glucose and OAS resulted in a more than additive induction of APR activity. Contrary to nitrate reductase, APR was also increased by glucose in N-deficient plants, indicating that this effect was independent of nitrate assimilation. [35S]-sulphate feeding experiments showed that the addition of glucose to dark-treated roots resulted in an increased incorporation of [35S] into thiols and proteins, which corresponded to the increased levels of APR activity. Under N-deficient conditions, glucose also increased thiol labelling, but did not increase the incorporation of label into proteins. These results demonstrate that (i) exogenously supplied glucose can replace the function of photoassimilates in roots; (ii) APR is subject to co-ordinated metabolic control by carbon metabolism; (iii) positive sugar signalling overrides negative signalling from nitrate assimilation in APR regulation. Furthermore, signals originating from nitrogen and carbon metabolism regulate APR synergistically.
Key words: Adenosine-5'-phosphosulphate, APS reductase, O-acetyl-L-serine, sulphate assimilation.
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