Skip Navigation


JXB Advance Access originally published online on May 13, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/388/1711    most recent
erg178v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Footitt, S.
Right arrow Articles by von Arnold, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Footitt, S.
Right arrow Articles by von Arnold, S.
Agricola
Right arrow Articles by Footitt, S.
Right arrow Articles by von Arnold, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 388, pp. 1711-1719, July 1, 2003
© 2003 Oxford University Press

Expression of the viviparous 1 (Pavp1) and p34cdc2 protein kinase (cdc2Pa) genes during somatic embryogenesis in Norway spruce (Picea abies [L.] Karst)

Received 27 January 2003; Accepted 23 March 2003

Steven Footitt*,, Mathieu Ingouff{dagger},, David Clapham and Sara von Arnold{ddagger},

Department of Plant Biology and Forest Genetics, Uppsala Genetic Centre, Swedish University of Agricultural Sciences, Box 7027, Uppsala 750-07, Sweden

* Present address: Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
{dagger} Present address: Laboratoire RDP, UMR 5667, ENS Lyon, 46 allée d‘Italie, F-69364 Lyon cedex 07, France.
{ddagger}To whom correspondence should be addressed. Fax: +46 018 673279. E-mail: sara.von.arnold{at}sgen.slu.se
Abbreviations: ABA, abscisic acid; HRH, high relative humidity; LP, liquid proliferation; NLS, nuclear localization signal; PCD, programmed cell death; PCR, polymerase chain reaction; PEG, polyethylene glycol; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription-polymerase chain reaction.

Detailed expression analysis of the Norway spruce (Picea abies [L.] Karst) Viviparous 1 (Pavp1) and p34cdc2 (cdc2Pa) genes was carried out during somatic embryogenesis. Pavp1, a gene associated with embryo development, was expressed in proliferating embryogenic suspension cultures in the absence of exogenous ABA. When somatic embryo formation was promoting by blocking proliferation, Pavp1 expression was reduced. During maturation, exogenous ABA induced increased Pavp1 expression, which peaked at the early cotyledonary stage of somatic embryogenesis. Following partial desiccation of mature somatic embryos at high relative humidity, Pavp1 expression persisted under germination conditions. Pavp1 expression was also detected in non-dormant immature male strobili and dormant terminal buds. These data confirm the functional conservation of Pavp1 during the evolution of seed plants and extend its function beyond the embryo. Cdc2Pa, a gene associated with the cell cycle, was up-regulated when the proliferation of embryogenic cells was blocked. Expression was again up-regulated in early embryogeny and again during germination. The implications of this up-regulation of cdc2Pa are discussed.

Key words: abi3, apoptosis, cdc2, dormancy, maturation, programmed cell death, somatic embryogenesis, spruce, vp1.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.