Skip Navigation


JXB Advance Access originally published online on May 28, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/388/1743    most recent
erg186v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lawson, T.
Right arrow Articles by Baker, N. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lawson, T.
Right arrow Articles by Baker, N. R.
Agricola
Right arrow Articles by Lawson, T.
Right arrow Articles by Baker, N. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 388, pp. 1743-1752, July 1, 2003
© 2003 Oxford University Press

The responses of guard and mesophyll cell photosynthesis to CO2, O2, light, and water stress in a range of species are similar

Received 20 December 2002; Accepted 7 April 2003

Tracy Lawson, Kevin Oxborough, James I. L. Morison*, and Neil R. Baker

Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, UK

* To whom correspondence should be addressed. Fax: +44 (0)1206 873416. E-mail: morisj{at}essex.ac.uk
Abbreviations: Ca, ambient CO2 concentration; F', chlorophyll fluorescence in the light-adapted state; Fm', chlorophyll fluorescence when PSII centres are maximally closed in the light-adapted state; Fo', chlorophyll fluorescence when PSII centres are maximally open in the light-adapted state; Fq', difference between F' and Fm'; Fq'/Fm', fluorescence parameter that provides an estimate of the operating efficiency of PSII photochemistry; Fq'/Fv', factor relating the operating and maximum efficiencies of PSII photochemistry; Fv', variable chlorophyll fluorescence (Fm'–Fo'); Fv'/Fm', fluorescence parameter that provides an estimate of the maximum efficiency of PSII photochemistry (when all PSII centres are open); PPFD, photosynthetic photon flux density; Rubisco, ribulose 1,5-bisphosphate carboxylase oxygenase; VPD, vapour pressure deficit.

High resolution chlorophyll a fluorescence imaging was used to compare the photosynthetic efficiency of PSII electron transport (estimated by Fq'/Fm') in guard cell chloroplasts and the underlying mesophyll in intact leaves of six different species: Commelina communis, Vicia faba, Amaranthus caudatus, Polypodium vulgare, Nicotiana tabacum, and Tradescantia albifora. While photosynthetic efficiency varied between the species, the efficiencies of guard cells and mesophyll cells were always closely matched. As measurement light intensity was increased, guard cells from the lower leaf surfaces of C. communis and V. faba showed larger reductions in photosynthetic efficiency than those from the upper surfaces. In these two species, guard cell photosynthetic efficiency responded similarly to that of the mesophyll when either light intensity or CO2 concentration during either measurement or growth was changed. In all six species, reducing the O2 concentration from 21% to 2% reduced guard cell photosynthetic efficiency, even for the C4 species A. caudatus, although the mesophyll of the C4 species did not show any O2 modulation of photosynthetic efficiency. This suggests that Rubisco activity is significant in the guard cells of these six species. When C. communis plants were water-stressed, the guard cell photosynthetic efficiency declined in parallel with that of the mesophyll. It was concluded that the photosynthetic efficiency in guard cells is determined by the same factors that determine it in the mesophyll.

Key words: Chlorophyll fluorescence, guard cell, mesophyll, photosynthesis, stomata, water stress.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
K. Omasa, A. Konishi, H. Tamura, and F. Hosoi
3D Confocal Laser Scanning Microscopy for the Analysis of Chlorophyll Fluorescence Parameters of Chloroplasts in Intact Leaf Tissues
Plant Cell Physiol., January 1, 2009; 50(1): 90 - 105.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Baroli, G. D. Price, M. R. Badger, and S. von Caemmerer
The Contribution of Photosynthesis to the Red Light Response of Stomatal Conductance
Plant Physiology, February 1, 2008; 146(2): 737 - 747.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. M. Messinger, T. N. Buckley, and K. A. Mott
Evidence for Involvement of Photosynthetic Processes in the Stomatal Response to CO2
Plant Physiology, February 1, 2006; 140(2): 771 - 778.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. von Caemmerer, T. Lawson, K. Oxborough, N. R. Baker, T. J. Andrews, and C. A. Raines
Stomatal conductance does not correlate with photosynthetic capacity in transgenic tobacco with reduced amounts of Rubisco
J. Exp. Bot., June 1, 2004; 55(400): 1157 - 1166.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.