JXB Advance Access originally published online on July 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal of Experimental Botany, Vol. 54, No. 389, pp. 1879-1887,
August 1, 2003
© 2003 Oxford University Press
Molecular cloning and expression analysis of a CONSTANS homologue, PnCOL1, from Pharbitis nil
Received 18 February 2003; Accepted 16 May 2003
1 Department of Life Science, Sogang University, Seoul 121-742, Korea
2 School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
3 Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea
* To whom correspondence should be addressed. Fax: +822 704 3601. E-mail: sungkim{at}sogang.ac.kr
The Arabidopsis CONSTANS (CO) gene is a key regulator of the long day (LD)-dependent flowering pathway and two CO homologous genes COL1 and COL2 are involved in the regulation of the circadian rhythm. In order to understand the role of CO and COL in short-day plants, a CO homologue, PnCOL1, was isolated and characterized from Japanese morning glory (Pharbitis nil). The deduced PnCOL1 protein of 386 amino acid residues contained two putative zinc finger motifs at the N-terminal region and a conserved CCT domain at the C-terminal region. The deduced amino acid sequence of PnCOL1 was 34% identical to that of PnCO, but 32%, 34%, and 34% identical to those of CO, COL1, and COL2, respectively. Expression of PnCOL1 was barely detected in the cotyledons of plants grown under continuous light (CL), but highly expressed in the cotyledons of plants grown under SD. Expression of PnCOL1 showed a pattern of circadian rhythm as well as daily oscillation. The overexpression of PnCOL1 by a 35S promoter did not overcome the late-flowering phenotype of Arabidopsis co mutants. The results provided in this study suggest that PnCOL1 may have a role in the circadian rhythm in Pharbitis nil.
Key words: cDNA, floral primordia, Pharbitis nil, PnCOL1, short-day plant.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. C. Zheng, D. Potter, and S. D. O'Neill Phytochrome gene expression and phylogenetic analysis in the short-day plant Pharbitis nil (Convolvulaceae): Differential regulation by light and an endogenous clock Am. J. Botany, July 1, 2009; 96(7): 1319 - 1336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Y. P. Chia, A. Muller, C. Jung, and E. S. Mutasa-Gottgens Sugar beet contains a large CONSTANS-LIKE gene family including a CO homologue that is independent of the early-bolting (B) gene locus J. Exp. Bot., July 1, 2008; 59(10): 2735 - 2748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hayama, B. Agashe, E. Luley, R. King, and G. Coupland A Circadian Rhythm Set by Dusk Determines the Expression of FT Homologs and the Short-Day Photoperiodic Flowering Response in Pharbitis PLANT CELL, October 1, 2007; 19(10): 2988 - 3000. [Abstract] [Full Text] [PDF]
|
|