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JXB Advance Access originally published online on June 18, 2003
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Journal of Experimental Botany, Vol. 54, No. 389, pp. 1997-1999, August 1, 2003
© 2003 Oxford University Press

Cloning and expression of a UDP-glucuronic acid decarboxylase gene in rice

Received 20 January 2003; Accepted 29 April 2003

Kiyoshi Suzuki1, Yasuhiko Suzuki2 and Shinichi Kitamura*,1

1 Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Gakuen-cho 1-1, Sakai, Osaka 599-8531, Japan
2 Division of Pathology, Osaka Prefectural Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka, Osaka 537-0025, Japan

* To whom correspondence should be addressed. Fax: +81 72 254 9458. E-mail: skita{at}biochem.osakafu-u.ac.jp

A cDNA fragment was cloned from rice immature seeds by the RT-PCR method. The deduced amino acid sequence of the cDNA showed a high degree of identity with UDP-D-glucuronic acid decarboxylase (UXS) from other plants and was most similar to the soluble UXS from Arabidopsis. The recombinant protein, expressed in an Escherichia coli system, catalysed the conversion of UDP-D-glucuronic acid to UDP-D-xylose, confirming that the gene encoded UXS. The uxs gene was expressed in mature, harvested rice seeds as well as in immature seeds 14 d post-anthesis, suggesting that the uxs gene is necessary at the beginning of the germination period. This is the first report of the cloning of the uxs gene from monocots.

Key words: Endosperm, gene expression, Oryza sativa cv. Nipponbare (monocots), UDP-glucuronic acid decarboxylase (UXS).


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