Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Trouverie, J.
Right arrow Articles by Prioul, J.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trouverie, J.
Right arrow Articles by Prioul, J.-L.
Agricola
Right arrow Articles by Trouverie, J.
Right arrow Articles by Prioul, J.-L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 390, pp. 2177-2186, September 1, 2003
© 2003 Oxford University Press

The role of abscisic acid in the response of a specific vacuolar invertase to water stress in the adult maize leaf

Received 8 April 2003; Accepted 10 June 2003

Jacques Trouverie1, Claudine Thévenot1, Jean-Paul Rocher1, Bruno Sotta2 and Jean-Louis Prioul*,1

1 Equipe Structure et Métabolisme des Plantes, Institut de Biotechnologie des Plantes (UMR-CNRS 8618), Bâtiment 630, Université Paris-Sud, 91405 ORSAY Cedex, France
2 Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes (UMR-CNRS 7632), Université Pierre et Marie Curie, 4 Place Jussieu, Tour 53 E5, Casier 156, 75252 Paris Cedex 05, France

* To whom correspondence should be addressed. Fax: +33 1 69 15 34 24. E-mail: prioul{at}ibp.u-psud.fr

Among the numerous molecular and physiological modifications induced by water deficit, one of the earliest events observed in maize mature leaves subjected to water deprivation was a strong enhancement of acid vacuolar invertase activity, which occurred before the classical reduction in gas exchange due to stomatal closure. The increase in invertase activity coincided with the rapid accumulation of glucose and fructose that reached 8-fold the control leaf value. In addition, acid vacuolar invertase activity appeared to be highly correlated with xylem sap ABA concentration. In order to investigate the nature of the relationship between ABA and invertase activity, and to disconnect ABA from a likely sucrose side-effect, excised leaves were supplied with ABA or sucrose. As a consequence of ABA supply, a peak in leaf ABA appeared 4 h later which was followed by an enhancement of vacuolar invertase activity. ABA supply also produced a second maximum in leaf ABA. The transcript level of the Ivr2 gene encoding one vacuolar invertase presented the same two peaks pattern as leaf ABA, with a 2 h lag. This response was specific since the other invertase genes were not responding. Thus, ABA appeared to be a powerful enhancer of the IVR2 vacuolar invertase activity and expression. In the present conditions, the addition of sucrose had no effect on the enzyme activity.

Key words: Abscisic acid, carbohydrates, vacuolar invertase, Ivr2, Rab 17, water stress, Zea mays.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
C. Fresneau, J. Ghashghaie, and G. Cornic
Drought effect on nitrate reductase and sucrose-phosphate synthase activities in wheat (Triticum durum L.): role of leaf internal CO2
J. Exp. Bot., August 30, 2007; (2007) erm150v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. S. Boyer and J. E. McLaughlin
Functional reversion to identify controlling genes in multigenic responses: analysis of floral abortion
J. Exp. Bot., January 1, 2007; 58(2): 267 - 277.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. S. Boyer and M. E. Westgate
Grain yields with limited water
J. Exp. Bot., November 1, 2004; 55(407): 2385 - 2394.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. M. Chaves and M. M. Oliveira
Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture
J. Exp. Bot., November 1, 2004; 55(407): 2365 - 2384.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.