JXB Advance Access originally published online on September 25, 2003
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Journal of Experimental Botany, Vol. 54, No. 392, pp. 2431-2437,
November 1, 2003
© 2003 Oxford University Press
Self-incompatibility (S) locus region of the mutated S6-haplotype of sour cherry (Prunus cerasus) contains a functional pollen S allele and a non-functional pistil S allele
Received 17 March 2003; Accepted 17 July 2003
1 Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto 606–8502, Japan
2 Department of Horticulture, Michigan State University, East Lansing, MI 48824, USA
* To whom correspondence should be addressed. Fax: +81 75 753 6497. E-mail: rtao{at}kais.kyoto-u.ac.jp
This study characterizes the S6m-haplotype, a mutated S6-haplotype with an altered HindIII cut site, of sour cherry (Prunus cerasus). Inheritance and pollination studies of S-haplotypes from reciprocal crosses between ‘Erdi Botermo’ (EB; S4S6mSa) and ‘Rheinische Schattenmorelle’ (RS; S6SaSbSc) revealed that the S6m-haplotype conferred unilateral incompatibility with a non-functional pistil component and a functional pollen component. Expression analyses of S6-RNase and SFB6, a candidate gene for pollen-S, in the S6m-haplotype showed that SFB6 was transcribed in EB pollen, but S6-RNase was not transcribed in EB styles. These results were consistent with data from the inheritance and pollination studies. Inverse PCR for the flanking regions of S6-RNase in the S6- and S6m-haplotypes revealed an approximately 2600 bp insertion present at approximately 800 bp upstream of the S6-RNase in the S6m-haplotype, which is responsible for the alternation of the HindIII cut site and a possible cause of inhibition of the transcription of S6-RNase. SFB6 was present downstream of S6-RNase in both the S6- and S6m-haplotypes and expressed in the same way, supporting the idea that SFB is a good candidate for pollen-S in Prunus.
Key words: Pistil determinant, pollen determinant, polyploidy, Prunus avium, Rosaceae, SFB, S-RNase.
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