JXB Advance Access originally published online on September 25, 2003
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Journal of Experimental Botany, Vol. 54, No. 392, pp. 2467-2477,
November 1, 2003
© 2003 Oxford University Press
Two 
-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development*
Received 3 March 2003; Accepted 17 July 2003
Department of Genetics, University of Melbourne, Victoria 3010, Australia
* The cDNA nucleotide sequences reported in this paper have been submitted to the GenBankTM/EMBL Nucleotide Sequence database with accession numbers AY243509 (AtASD1) and AY243510 (AtASD2). To whom correspondence should be addressed. Fax: +61 3 8344 5139. E-mail: ccobbett{at}unimelb.edu.au
Abbreviations: CAZY, carbohydrate-active enzyme; RT-PCR, reverse transcriptase polymerase chain reaction.
Glycosyl hydrolases are important mediators of plant cell wall modification during plant development. These enzymes catalyse the hydrolytic release of specific sugars, such as L-arabinose, from the polysaccharide-rich cell wall matrix. The cloning and expression analysis of two genes, AtASD1 and AtASD2, encoding putative 
-L-arabinofuranosidases in Arabidopsis thaliana are reported here. AtASD1 and AtASD2 identities were assigned on the basis of homology to plant and microbial family 51 glycoside hydrolases. Using RT-PCR, RNA gel blot analysis and reporter gene expression analysis, AtASD1 and AtASD2 were shown to have different developmental expression profiles. High levels of AtASD1 promoter activity are present in multiple tissues during vegetative and reproductive growth. AtASD1 expression is particularly intense in zones of cell proliferation, the vascular system, developing and regressing floral tissues, and floral abscission zones. By comparison, AtASD2 expression is limited to the vasculature of older root tissue and to some floral organs and floral abscission zones.
Key words: Abscission, Arabidopsis, arabinose, expression, gene structure, glycoside hydrolases, GUS (ß-glucuronidase), vegetative growth.
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