Skip Navigation


JXB Advance Access originally published online on September 9, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/392/2529    most recent
erg270v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ranieri, A.
Right arrow Articles by Soldatini, G. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ranieri, A.
Right arrow Articles by Soldatini, G. F.
Agricola
Right arrow Articles by Ranieri, A.
Right arrow Articles by Soldatini, G. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 392, pp. 2529-2540, November 1, 2003
© 2003 Oxford University Press

Early production and scavenging of hydrogen peroxide in the apoplast of sunflower plants exposed to ozone

Received 11 February 2003; Accepted 17 July 2003

A. Ranieri*,1, A. Castagna1, J. Pacini1, B. Baldan2, A. Mensuali Sodi3 and G. F. Soldatini1

1 Department of Agricultural Chemistry and Biotechnology, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy
2 Department of Biology, University of Padova, Padova, I-35131, Italy
3 Sant’Anna School of University Studies and Doctoral Research, Pisa, I-56124, Italy

* To whom correspondence should be addressed. Fax: +39 50 598614. E-mail: aranieri{at}agr.unipi.it
Abbreviations: APX, ascorbate peroxidase; CAB, Na-cacodylate buffer; CB, cell wall covalently-bound fraction; DPI, diphenylene iodonium; DTT, dithiothreitol; HEPES, N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid; IB, cell wall ionically-bound fraction; IWF, intercellular washing fluid; MOPS, 3-(N-morpholino)propanesulphonic acid; NAD(P)H PODs, H2O2 producing NAD(P)H-oxidizing peroxidases; PM, plasma membrane; PODs, peroxidases; pCMB, p-chloromercuribenzoate; ROS, reactive oxygen species; Syr-PODs, H2O2 scavenging syringaldazine-reducing peroxidases; TBARS, thiobarbituric acid reactive substances.

The present work set out to define the processes involved in the early O3-induced H2O2 accumulation in sunflower plants exposed to a single pulse of 150 ppb of O3 for 4 h. Hydrogen peroxide accumulation only occurred in the apoplast and this temporally coincided with the fumigation period. The inhibitor experiments suggested that both the plasma membrane-bound NAD(P)H oxidase complex and cell-wall NAD(P)H PODs contributed to H2O2 generation. To investigate the mechanisms responsible for O3-induced H2O2 accumulation further, both production and scavenging of H2O2 were investigated in the extracellular matrix after subcellular fractionation. The results indicated that H2O2 accumulation is a complex and highly regulated event requiring the time-dependent stimulation and down-regulation of differently located enzymes, some of which are involved in H2O2 generation and degradation, not only during the fumigation period but also in the subsequent recovery period in non-polluted air. Owing to the possible interplay between H2O2 and ethylene, the time-course of ethylene emission was analysed too. Ethylene was rapidly emitted following O3 exposure, but it declined to control values as early as after 4 h of exposure. The early contemporaneous detection of increased ethylene and H2O2 levels after 30 min of exposure does not allow a clear temporal relationship between these two signalling molecules to be established.

Key words: Ascorbate peroxidase, ethylene, hydrogen peroxide, NAD(P)H oxidase, ozone, peroxidases, reactive oxygen species, signal transduction, sunflower (Helianthus annuus L.).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
T. Jubany-Mari, S. Munne-Bosch, M. Lopez-Carbonell, and L. Alegre
Hydrogen peroxide is involved in the acclimation of the Mediterranean shrub, Cistus albidus L., to summer drought
J. Exp. Bot., January 1, 2009; 60(1): 107 - 120.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P Diaz-Vivancos, M Rubio, V Mesonero, P. Periago, A Ros Barcelo, P Martinez-Gomez, and J. Hernandez
The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection
J. Exp. Bot., November 1, 2006; 57(14): 3813 - 3824.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. d. C. Cordoba-Pedregosa, J. M. Villalba, F. Cordoba, and J. A. Gonzalez-Reyes
Changes in intracellular and apoplastic peroxidase activity, ascorbate redox status, and root elongation induced by enhanced ascorbate content in Allium cepa L.
J. Exp. Bot., February 1, 2005; 56(412): 685 - 694.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. H. Lee, A. P. Singh, and G. C. Chung
Rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decreases in water transport
J. Exp. Bot., August 1, 2004; 55(403): 1733 - 1741.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.