Skip Navigation


JXB Advance Access originally published online on December 12, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/395/213    most recent
erh007v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (25)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Seki, M.
Right arrow Articles by Shinozaki, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seki, M.
Right arrow Articles by Shinozaki, K.
Agricola
Right arrow Articles by Seki, M.
Right arrow Articles by Shinozaki, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 395, pp. 213-223, January 1, 2004
© 2004 Oxford University Press

Signalling in Abiotic Stress

RIKEN Arabidopsis full-length (RAFL) cDNA and its applications for expression profiling under abiotic stress conditions

Received 21 March 2003; Accepted 22 July 2003

Motoaki Seki1,2, Masakazu Satou1, Tetsuya Sakurai1, Kenji Akiyama1, Kei Iida1, Junko Ishida1, Maiko Nakajima1, Akiko Enju1, Mari Narusaka1, Miki Fujita1, Youko Oono2,3, Ayako Kamei2, Kazuko Yamaguchi-Shinozaki4 and Kazuo Shinozaki1,2,*

1 Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
2 Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan
3 Master’s Program in Biosystem Studies, University of Tsukuba, Tennoudai, Tsukuba, Ibaraki, 305-0074, Japan
4 Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS),Ministry of Agriculture, Forestry, and Fisheries, 2-1 Ohwashi, Tsukuba 305-0074, Japan

* To whom correspondence should be addressed. Fax: +81 298 36 9060. E-mail: sinozaki{at}rtc.riken.go.jp
Abbreviations: ABA, abscisic acid ; bHLH, basic helix-loop-helix; bp, base pairs; cab, chlorophyll a/b-binding protein; CaMV, cauliflower mosaic virus; CBF, C-repeat-binding factor; cos, constitutive expression of osmotically responsive genes; DRE, dehydration-responsive element; DREB, DRE-binding protein; dsRNAi, double-stranded RNA interference; ERD, early responsive to dehydration; ERF, ethylene-responsive element binding factor; ESTs, expressed sequence tags; HD-ZIP, homeodomain-leucine zipper; hos, high expression of osmotically responsive genes; JA, jasmonic acid; KIN, cold-inducible; LEA, late embryogenesis abundant; los, low expression of osmotically responsive genes; LUC, firefly luciferase; P5C, {Delta}1-pyrroline-5-carboxylate; Pro, proline; ProDH, proline dehydrogenase; RAFL, RIKEN Arabidopsis full-length; rbcs, ribulose 1,5-bisphosphate carboxylase small subunit; RFO, raffinose family oligosaccharides; RD, responsive to dehydration; sos, salt overly sensitive.

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. 155 144 RIKEN Arabidopsis full-length (RAFL) cDNA clones were isolated. The 3'-end expressed sequence tags (ESTs) of all 155 144 RAFL cDNAs were clustered into 14 668 non-redundant cDNA groups, about 60% of predicted genes. The sequence database of the RAFL cDNAs is useful for promoter analysis and the correct annotation of predicted transcription units and gene products. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. RAFL cDNA microarrays were prepared, containing independent full-length cDNA groups for analysing the expression profiles of genes under various stress- and hormone-treatment conditions and in various mutants and transgenic plants. In this review, recent progress on transcriptome analysis using the RAFL cDNA microarray is highlighted.

Key words: cDNA microarray, cold stress, drought stress, full-length cDNA, gene expression, high-salinity stress, RAFL cDNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
S. Komatsu, H. Konishi, and M. Hashimoto
The proteomics of plant cell membranes
J. Exp. Bot., January 1, 2007; 58(1): 103 - 112.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M Senthil-Kumar and M Udayakumar
High-throughput virus-induced gene-silencing approach to assess the functional relevance of a moisture stress-induced cDNA homologous to lea4
J. Exp. Bot., July 1, 2006; 57(10): 2291 - 2302.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Pourcel, J.-M. Routaboul, L. Kerhoas, M. Caboche, L. Lepiniec, and I. Debeaujon
TRANSPARENT TESTA10 Encodes a Laccase-Like Enzyme Involved in Oxidative Polymerization of Flavonoids in Arabidopsis Seed Coat
PLANT CELL, November 1, 2005; 17(11): 2966 - 2980.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Bertone and M. Snyder
Prospects and Challenges in Proteomics
Plant Physiology, June 1, 2005; 138(2): 560 - 562.
[Full Text] [PDF]

Home page
 ScienceHome page
J. Cheng, P. Kapranov, J. Drenkow, S. Dike, S. Brubaker, S. Patel, J. Long, D. Stern, H. Tammana, G. Helt, et al.
Transcriptional Maps of 10 Human Chromosomes at 5-Nucleotide Resolution
Science, May 20, 2005; 308(5725): 1149 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. P. Brandt
Microgenomics: gene expression analysis at the tissue-specific and single-cell levels
J. Exp. Bot., February 1, 2005; 56(412): 495 - 505.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. A. Bray
Genes commonly regulated by water-deficit stress in Arabidopsis thaliana
J. Exp. Bot., November 1, 2004; 55(407): 2331 - 2341.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.