JXB Advance Access originally published online on January 30, 2004
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Journal of Experimental Botany, Vol. 55, No. 397, pp. 585-594, March 1, 2004
© 2004 Oxford University Press
Cell and Molecular Biology, Biochemistry and Molecular Physiology |
Effect of salt and osmotic stresses on the expression of genes for the vacuolar H+-pyrophosphatase, H+-ATPase subunit A, and Na+/H+ antiporter from barley*
Received 16 May 2003; Accepted 13 November 2003
1 National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan
2 Institute of Biological Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan
3 Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
* The nucleotide sequences reported in this paper have been submitted to the DDBJ/EMBL/GenBank with accession numbers AB032839 (HVP1), AB032840 (HvVHA-A), and AB089197 (HvNHX1). To whom correspondence should be addressed. Fax: +81 29 838 8347. E-mail: fukuda{at}affrc.go.jp Present address: Brewing Group, Takasaki Plant, Kirin Brewery Company, Limited, Miyahara-cho, Takasaki, Gunma 370-1202, Japan.
Abbreviations: ORF, open reading frame; RACE, rapid amplification of cDNA ends; UTR, untranslated region; V-ATPase, vacuolar H+-ATPase; V-PPase, vacuolar H+-inorganic pyrophosphatase.
Two cDNA clones encoding vacuolar H+-inorganic pyrophosphatase (HVP1 and HVP10), one clone encoding the catalytic subunit (68 kDa) of vacuolar H+-ATPase (HvVHA-A), and one clone encoding vacuolar Na+/H+ antiporter (HvNHX1) were isolated from barley (Hordeum vulgare), a salt-tolerant crop. Salt stress increased the transcript levels of HVP1, HVP10, HvVHA-A, and HvNHX1, and osmotic stress also increased the transcript levels of HVP1 and HvNHX1 in barley roots. The transcription of HVP1 in response to salt stress was regulated differently from that of HVP10. In addition, the HVP1 expression changed in a pattern similar to that of HvNHX1 expression. These results indicate that the expression of HVP1 is co-ordinated with that of HvNHX1 in barley roots in response to salt and osmotic stresses.
Key words: Barley (Hordeum vulgare), Na+/H+ antiporter, salt stress, vacuolar H+-ATPase (EC 3.6.1.3), vacuolar H+-inorganic pyrophosphatase (EC 3.6.1.1).
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