Skip Navigation


JXB Advance Access originally published online on February 27, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/398/847    most recent
erh093v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Cuadrado, A.
Right arrow Articles by de la Torre, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cuadrado, A.
Right arrow Articles by de la Torre, C.
Agricola
Right arrow Articles by Cuadrado, A.
Right arrow Articles by de la Torre, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 398, pp. 847-854, April 1, 2004
© 2004 Oxford University Press

Cell and Molecular Biology, Biochemistry and Molecular Physiology

Genome remodelling in three modern S. officinarumxS. spontaneum sugarcane cultivars

6 November 2003; 22 December 2003

A. Cuadrado1,*, R. Acevedo2, S. Moreno Díaz de la Espina3, N. Jouve1 and C. de la Torre3

1 Department of Cell Biology and Genetics, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain
2 Instituto de Investigaciones de la Caña de Azúcar, Carretera a Central Martinez Prieto Km 2 1/2, Boyeros, Ciudad de La Habana, Cuba
3 Centro de Investigaciones Biológicas, CSIC, Velázquez, 144. E-28006 Madrid, Spain

* To whom correspondence should be addressed. Fax: +34 91 8854758. E-mail: angeles.cuadrado{at}uah.es

This study provides evidence that nuclear and chromosome remodelling has taken place in sugarcane, a vegetative crop with a complex genome derived from interspecific hybridizations between Saccharum officinarum and S. spontaneum. Detailed knowledge on the chromosomal compositions of the three clones analysed was acquired. (1) All hybrid cultivars were found to be aneuploid, affecting both parental genomes (having chromosomes in addition to full genomes), with chromosome numbers from 2n=102–106 in My5514 and up to 2n=113–117 in C236-51. (2) Comparative in situ hybridization showed that about 16% of these chromosomes are inherited from S. spontaneum and less than 5% are recombinant or translocated chromosomes containing sequences of both S. officinarum and S. spontaneum. (3) Differences between the observed DNA contents (estimated by flow cytometry) and those expected from the number of chromosomes, allowed the introgression of additional S. spontaneum or S. officinarum DNA pieces into the B42231 and C236-51 cultivars to be estimated. (4) Size heterogeneity between S. officinarum homologous chromosomes carrying the 18S–5.8S–25S and 5S ribosomal genes (identified by FISH with pTa71 and pTa794, respectively) confirms remodelling occurred by chromosomal interchange events, at least in these homologous chromosomes. (5) Simultaneous visualization of nucleoli and NORs showed that all 18S–5.8S–25S loci were potentially functional in the three clones, independent of their origin and size.

Key words: Flow cytometry, genome remodelling, in situ hybridization, intergenomic recombination, nucleolar competition, ribosomal genes, Saccharum spp.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Crop Sci.Home page
X.-H. Wang, Q.-H. Yang, F.-S. Li, L.-L. He, and S.-C. He
Molecular Identification of Saccharum spp. x Erianthus fulvus Hybrids Using Sequence-Characterized Amplified Region Markers
Crop Sci., May 11, 2009; 49(3): 864 - 870.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. Le Cunff, O. Garsmeur, L. M. Raboin, J. Pauquet, H. Telismart, A. Selvi, L. Grivet, R. Philippe, D. Begum, M. Deu, et al.
Diploid/Polyploid Syntenic Shuttle Mapping and Haplotype-Specific Chromosome Walking Toward a Rust Resistance Gene (Bru1) in Highly Polyploid Sugarcane (2n ~ 12x ~ 115)
Genetics, September 1, 2008; 180(1): 649 - 660.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.