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JXB Advance Access originally published online on March 12, 2004
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Journal of Experimental Botany, Vol. 55, No. 398, pp. 909-918, April 1, 2004
© 2004 Oxford University Press

Regulation of Growth, Development and Whole Organism Physiology

Pectin esterase gene family in strawberry fruit: study of FaPE1, a ripening-specific isoform*

Received 10 September 2003; Accepted 20 January 2004

Cristina Castillejo1, José Ignacio de la Fuente1, Pietro Iannetta2, Miguel Ángel Botella1 and Victoriano Valpuesta1,{dagger}

1 Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga,Campus de Teatinos, E-29071 Málaga, Spain
2 Ecosystem Management and Biotechnology, Scottish Crop Research Institute, Dundee DD2 4DN, UK

* The sequences reported in this paper have been deposited in the GenBank database under the accession numbers AY324809 (FaPE1), AY357182 (FaPE2), AY357183 (FaPE3), and AY357184 (FaPE4).
{dagger} To whom correspondence should be addressed. Fax: +34 952 13 19 32. E-mail: valpuesta{at}uma.es
Abbreviations: PE, pectin esterase; HGA, homogalacturonic acid.

Pectin esterases (PE, EC 3.1.1.11) catalyse the demethylation of pectin. As a result of its activity, structural interactions among cell wall components during cell wall turnover and loosening are affected. In plants, PEs are typically encoded by a gene family. This family has been studied in strawberry (Fragariaxananassa Duch.) in order to investigate the role of distinct PE genes during fruit ripening and senescence. By a combination of a PCR-based library screening and RT-PCR four different strawberry PE cDNAs, termed FaPE1 to FaPE4, have been isolated. Differential expression of each FaPE gene in various organs and during fruit development was revealed by northern blot. FaPE1 is specifically expressed in fruit, showing an increasing expression during the ripening process up to a maximum in the turning stage. Concerning hormone regulation, auxin treatment increased FaPE1 mRNA levels in green fruit, whereas exogenous ethylene decreased FaPE1 mRNA levels in ripe and senescing fruits. It is proposed that this repression of FaPE1 expression could be involved in textural changes occurring during fruit senescence.

Key words: Auxin, cell wall, ethylene, fruit ripening, pectin esterase, strawberry.


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