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JXB Advance Access originally published online on February 27, 2004
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Journal of Experimental Botany, Vol. 55, No. 398, pp. 955-956, April 1, 2004
© 2004 Oxford University Press

GENE NOTE

Cloning and expression of cytosolic phosphoglycerate kinase from pea (Pisum sativum L.)

Received 25 July 2003; Accepted 16 December 2003

David C. Brice*, John A. Bryant{dagger}, Gerardas Dambrauskas{ddagger}, Suzanne C. Drury and Jennifer A. Littlechild

Department of Biological Sciences, University of Exeter, Exeter EX4 4PS, UK

* Present address: John Innes Centre for Plant Science Research, Colney Lane, Norwich NR4 7UH, UK.
{dagger} To whom correspondence should be addressed. Fax: +44 (0)1392 264668. J.A.Bryant{at}ex.ac.uk
{ddagger} Present address: Department of Biochemistry, North Carolina State University, Raleigh, NC 27695, USA.
§ Present address: Molecular Toxicology Laboratory, Imperial College School of Medicine, London SW7 2AZ, UK.
Present address: Director, Henry Wellcome Centre for Biocatalysis, University of Exeter, Exeter EX4 4QD, UK.

In common with several other respiratory and photosynthetic enzymes, a sub-population of cytosolic phosphoglycerate kinase (PGK) occurs in the nucleus in pea leaves and shoots. The full-length cDNA encoding pea cytosolic PGK has been cloned and sequenced, revealing not only the PGK ‘signature’ but also a nuclear localization signal (NLS). A translational fusion of PGK and GFP was used to transform tobacco BY-2 cells resulting in GFP locating to the cell nuclei.

Key words: GFP, moonlighting proteins, nucleus, pea, phosphoglycerate kinase, Pisum sativum.


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