JXB Advance Access originally published online on April 23, 2004
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Journal of Experimental Botany, Vol. 55, No. 400, pp. 1157-1166, May 1, 2004
© 2004 Oxford University Press
Limitations to Photosynthetic Performance |
Stomatal conductance does not correlate with photosynthetic capacity in transgenic tobacco with reduced amounts of Rubisco
Received 5 November 2003; Accepted 10 February 2004
1 Molecular Plant Physiology Group, Research School of Biological Sciences, Australian National University, Canberra, ACT 2601, Australia
2 Department of Biological Sciences University of Essex, Colchester CO4 3SQ, UK
* To whom correspondence should be addressed. Fax +61 2 6125 5075. E-mail: Susanne.Caemmerer{at}anu.edu.au
Abbreviations: A, CO2 assimilation rate; Ca, ambient CO2 concentration; Ci, intercellular CO2 concentration, F', steady-state fluorescence during photosynthesis in the light; Fm maximun fluorescence of a dark-adapted leaf after a saturating light pulse; Fm', maximum fluorescence of a light-adapted leaf after a saturating light pulse; Fo, minimal fluorescence yield of a dark-adapted leaf; Fq'/Fm' = (Fm' – F')/Fm, quantum yield of electron flow to PSII; Fq'/Fv' = (Fm'– F')/(Fm – Fo), photochemical quenching; PSII, photosystem II; RuBP, D-ribulose-1,5-bisphosphate; Rubisco, RuBP carboxylase/oxygenase; g, stomatal conductance; I, irradiance; NPQ = (Fm – Fm')/Fm, Stern–Volmer non-photochemical quenching.
High-resolution imaging of chlorophyll a fluorescence from intact tobacco leaves was used to compare the quantum yield of PSII electron transport in the chloroplasts of guard cells with that in the underlying mesophyll cells. Transgenic tobacco plants with reduced amounts of Rubisco (anti-Rubisco plants) were compared with wild-type tobacco plants. The quantum yield of PSII in both guard cells and underlying mesophyll cells was less in anti-Rubisco plants than in wild-type plants, but closely matched between the two cell types regardless of genotype. CO2 assimilation rates of anti-Rubisco plants were 4.4 µmol m–2 s–1 compared with 17.3 µmol m–2 s–1 for the wild type, when measured at a photon irradiance of 1000 µmol m–2 s–1 and ambient CO2 of 380 µmol mol–1. Despite the large difference in photosynthetic capacity between the anti-Rubisco and wild-type plants, there was no discernible difference in the rate of stomatal opening, steady-state stomatal conductance or response of stomatal conductance to ambient CO2 concentration. These data demonstrate clearly that the commonly observed correlation between photosynthetic capacity and stomatal conductance can be disrupted in the long term by manipulation of photosynthetic capacity via antisense RNA technology. It was concluded that stomatal conductance is not directly determined by the photosynthetic capacity of guard cells or the leaf mesophyll.
Key words: Chlorophyll fluorescence, guard cell, Rubisco, stomatal conductance, tobacco.
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