Skip Navigation


JXB Advance Access originally published online on April 23, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/400/1195    most recent
erh145v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Oxborough, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oxborough, K.
Agricola
Right arrow Articles by Oxborough, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 400, pp. 1195-1205, May 1, 2004
© 2004 Oxford University Press

Electron Transport Processes

Imaging of chlorophyll a fluorescence: theoretical and practical aspects of an emerging technique for the monitoring of photosynthetic performance

Received 12 January 2004; Accepted 12 March 2004

Kevin Oxborough*

Department of Biological Sciences, John Tabor Laboratories, University of Essex, Colchester, Essex CO4 3SQ, UK

* Fax: +44 (0)1206 873416. E-mail: koxbor{at}essex.ac.uk
Abbreviations: adc, analogue to digital converter; CCD, charge coupled device; Chl, chlorophyll; F', Chl a fluorescence level at any point between F'o and F'm; Fm, maximal Chl a fluorescence level from a dark-adapted sample; F'm, maximal Chl a fluorescence level from sample in light; Fo, minimal Chl a fluorescence level from a dark-adapted sample; F'o, minimal Chl a fluorescence level of sample in light; F'q, difference in Chl a fluorescence between F'm and F' (F'q = F'm – F'); Fv, variable Chl a fluorescence level from a dark-adapted sample (Fv = Fm – Fo); F'v, variable Chl a fluorescence level from a light-adapted sample (F'v = F'm – F'o); PSII (I), Photosystem II (I); QA, primary quinone acceptor of PSII.

The development of chlorophyll (Chl) a fluorescence imaging systems has greatly increased the versatility of Chl a fluorometry as a non-invasive technique for the investigation of photosynthesis in plants and algae. For example, systems that image at the microscopic level have made it possible to measure PSII photochemical efficiencies from chloroplasts within intact leaves and from individual algal cells within mixed populations, while systems that image over much larger areas have been used to investigate heterogeneous patterns of photosynthetic performance across leaves and in screening programmes that image tens or even hundreds of plants simultaneously. In addition, it is now practical to use fluorescence imaging systems as real-time, multi-channel fluorometers, which can be used to record continuous fluorescence traces from multiple leaves, plants, or algal cells. This paper discusses some of the theoretical and practical issues associated with the imaging of Chl a fluorescence and with Chl a fluorometry in general. This discussion includes a review of the most commonly used Chl a fluorescence parameters.

Key words: Chlorophyll fluorescence, imaging, photochemistry, photosynthesis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
P. D. Nabity, J. A. Zavala, and E. H. DeLucia
Indirect suppression of photosynthesis on individual leaves by arthropod herbivory
Ann. Bot., February 1, 2009; 103(4): 655 - 663.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Lawson, S. Lefebvre, N. R. Baker, J. I. L. Morison, and C. A. Raines
Reductions in mesophyll and guard cell photosynthesis impact on the control of stomatal responses to light and CO2
J. Exp. Bot., October 1, 2008; 59(13): 3609 - 3619.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Berger, A. K. Sinha, and T. Roitsch
Plant physiology meets phytopathology: plant primary metabolism and plant pathogen interactions
J. Exp. Bot., December 1, 2007; 58(15-16): 4019 - 4026.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Berger, Z. Benediktyova, K. Matous, K. Bonfig, M. J. Mueller, L. Nedbal, and T. Roitsch
Visualization of dynamics of plant-pathogen interaction by novel combination of chlorophyll fluorescence imaging and statistical analysis: differential effects of virulent and avirulent strains of P. syringae and of oxylipins on A. thaliana
J. Exp. Bot., March 1, 2007; 58(4): 797 - 806.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Chaerle, I. Leinonen, H. G. Jones, and D. Van Der Straeten
Monitoring and screening plant populations with combined thermal and chlorophyll fluorescence imaging
J. Exp. Bot., March 1, 2007; 58(4): 773 - 784.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Y. Tang, R. E. Zielinski, A. R. Zangerl, A. R. Crofts, M. R. Berenbaum, and E. H. DeLucia
The differential effects of herbivory by first and fourth instars of Trichoplusia ni (Lepidoptera: Noctuidae) on photosynthesis in Arabidopsis thaliana
J. Exp. Bot., February 1, 2006; 57(3): 527 - 536.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. S. Quilliam, P. J. Swarbrick, J. D. Scholes, and S. A. Rolfe
Imaging photosynthesis in wounded leaves of Arabidopsis thaliana
J. Exp. Bot., January 1, 2006; 57(1): 55 - 69.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. A. Coupe, B. G. Palmer, J. A. Lake, S. A. Overy, K. Oxborough, F. I. Woodward, J. E. Gray, and W. P. Quick
Systemic signalling of environmental cues in Arabidopsis leaves
J. Exp. Bot., January 1, 2006; 57(2): 329 - 341.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.