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JXB Advance Access originally published online on May 7, 2004
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Journal of Experimental Botany, Vol. 55, No. 400, pp. 1221-1230, May 1, 2004
© 2004 Oxford University Press

Photosynthetic Gene Expression

Responses of primary and secondary metabolism to sugar accumulation revealed by microarray expression analysis of the Arabidopsis mutant, pho3

Received 26 November 2003; Accepted 4 March 2004

Julie C. Lloyd* and Oksana V. Zakhleniuk

Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK

* To whom correspondence should be addressed. Fax: +44 1206 872592. E-mail: lloyj{at}essex.ac.uk

The Arabidopsis mutant pho3 accumulates sucrose and other carbohydrates to high levels, providing a means of investigating the genomic response to sucrose accumulation using microarray analysis. Wild-type and mutant plants were grown in soil to the mature rosette stage for the analysis of gene expression using the Affymetrix ATH1 chip, containing more than 22 500 probe sets. Small, but significant, decreases were observed in the expression of many genes encoding enzymes and regulatory proteins involved in primary carbon assimilation, suggesting that, in mature leaves of Arabidopsis, there is limited feedback regulation on gene expression by sugars. The study revealed a striking increase in the expression of the plastid glucose 6-phosphate/phosphate translocator, characteristically expressed only in heterotrophic tissues. This indicated a change in the nature of metabolite exchange between the plastid and the cytosol in the pho3 mutant. The expression of enzymes of starch synthesis also increased significantly. Very large increases were observed in the expression of transcription factors and enzymes involved in anthocyanin biosynthesis. This finding reinforces the emerging picture of an important role for primary metabolism in regulating secondary metabolism.

Key words: Anthocyanin, Arabidopsis thaliana, GPT, MYB proteins, primary metabolism, suc2, sucrose, sugar signalling.


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