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JXB Advance Access originally published online on March 26, 2004
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Journal of Experimental Botany, Vol. 55, No. 400, pp. 1231-1244, May 1, 2004
© 2004 Oxford University Press

Photosynthetic Carbon Fluxes

Using mutants to probe the in vivo function of plastid envelope membrane metabolite transporters

Received 12 September 2003; Accepted 18 December 2003

Andreas P. M. Weber*, Jörg Schneidereit and Lars M. Voll

Michigan State University, Department of Plant Biology, East Lansing, MI, 48824, USA

* To whom correspondence should be addressed. Fax: +1 517 432 5294. E-mail: aweber{at}msu.edu

During the last 15 years, much progress has been made in discovering genes encoding solute transporters of the inner plastid envelope membrane. For example, genes encoding transporters for phosphorylated intermediates, dicarboxylates, adenine nucleotides, inorganic anions, and monosaccharides have been cloned. In many cases, the corresponding proteins have been expressed in recombinant host systems for further functional studies, thus allowing detailed in vitro characterization of transporter properties. Knowledge of the gene sequences encoding these transporters have allowed reverse-genetic approaches to study transporter function in vivo. Antisense repression and T-DNA insertion mutagenesis have provided a range of transgenic and mutant plants in which the activity of specific plastid envelope transporters are massively decreased or abolished. Plants with altered transporter activities represent excellent tools to probe the in vivo function of these transporters. Moreover, changing the permeability of the plastid envelope membrane permits the targeted manipulation of subcellular metabolite pools.

Key words: Antisense, envelope membrane, gene knockout, membrane transport, metabolite transport, mutant, plastid, translocator.


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