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JXB Advance Access originally published online on May 7, 2004
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Journal of Experimental Botany, Vol. 55, No. 401, pp. 1315-1323, June 1, 2004
© 2004 Oxford University Press

RESEARCH PAPER

Isolation of cucumber CsARF cDNAs and expression of the corresponding mRNAs during gravity-regulated morphogenesis of cucumber seedlings

Received 9 December 2003; Accepted 2 March 2004

Yuko Saito1, Seiji Yamasaki1,*, Nobuharu Fujii1,{dagger}, Gretchen Hagen2, Tom Guilfoyle2 and Hideyuki Takahashi1

1 Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan
2 Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, Missouri 65211, USA

* Present address: Fukuoka University of Education, 1-1 Akamabunkyomachi, Munakata, Fukuoka 811-4192, Japan.
{dagger} To whom correspondence should be addressed. Fax: +81 22 723 8218. E-mail: nobuharu{at}ige.tohoku.ac.jp
Abbreviations: ARF, auxin response factor; ORF, open reading frame; DBD, DNA-binding domain; CTD, carboxyl-terminal domain.

Cucumber seedlings show positive gravitropism and bend in the transition zone between the hypocotyl and the root. The peg, a specialized protuberance, develops on the concave side of the bending transition zone. Auxin and the mRNA of an auxin-inducible gene (CsIAA1) isolated from cucumber are differentially accumulated across the transition zone during the gravity-regulated peg formation. In this study, five cDNAs of Auxin Response Factors (ARFs) from cucumber were isolated and their mRNA accumulation was compared with that of CsIAA1. The tissue specificity of CsARF2 mRNA accumulation was similar to that of CsIAA1. Because the structural character of CsARF2 predicts that it is a transcriptional activator, CsARF2 may be involved in the activation of CsIAA1 transcription, which plays a role in gravity-regulated peg formation. Neither gravity nor auxin affected mRNA accumulation of five CsARFs including CsARF2, suggesting that CsARF2 may be regulated at a post-transcriptional level to induce the asymmetric expression of the CsIAA1 gene in response to gravistimulation and auxin in cucumber seedlings.

Key words: Aux/IAA, auxin response factors (ARFs), Cucumis sativus, gravity, peg.


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