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JXB Advance Access originally published online on May 21, 2004
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Journal of Experimental Botany, Vol. 55, No. 401, pp. 1335-1341, June 1, 2004
© 2004 Oxford University Press

RESEARCH PAPER

Molecular mapping of a gene responsible for Al-activated secretion of citrate in barley

Received 14 January 2004; Accepted 16 March 2004

Jian Feng Ma1,*, Sakiko Nagao1, Kazuhiro Sato2, Hiroyuki Ito2, Jun Furukawa1 and Kazuyoshi Takeda2

1 Faculty of Agriculture, Kagawa University, Ikenobe 2393, Miki-cho, Kita-gun, Kagawa 761-0795, Japan
2 Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan

* To whom correspondence should be addressed. Fax: +81 87 891 3137. E-mail: maj{at}ag.kagawa-u.ac.jp
Abbreviations: QTL, quantitative trait locus; RFLP, restriction fragment length polymorphism.

Aluminium (Al) toxicity is an important limitation to barley (Hordeum vulgare L.) on acid soil. Al-resistant cultivars of barley detoxify Al externally by secreting citrate from the roots. To link the genetics and physiology of Al resistance in barley, genes controlling Al resistance and Al-activated secretion of citrate were mapped. An analysis of Al-induced root growth inhibition from 100 F2 seedlings derived from an Al-resistant cultivar (Murasakimochi) and an Al-sensitive cultivar (Morex) showed that a gene associated with Al resistance is localized on chromosome 4H, tightly linked to microsatellite marker Bmag353. Quantitative trait locus (QTL) analysis from 59 F4 seedlings derived from an F3 plant heterozygous at the region of Al resistance on chromosome 4H showed that a gene responsible for the Al-activated secretion of citrate was also tightly linked to microsatellite marker Bmag353. This QTL explained more than 50% of the phenotypic variation in citrate secretion in this population. These results indicate that the gene controlling Al resistance on barley chromosome 4H is identical to that for Al-activated secretion of citrate and that the secretion of citrate is one of the mechanisms of Al resistance in barley. The identification of the microsatellite marker associated with both Al resistance and citrate secretion provides a valuable tool for marker-assisted selection of Al-resistant lines.

Key words: Aluminium, barley, citrate secretion, gene mapping, microsatellite marker, resistance.


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