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Journal of Experimental Botany, Vol. 55, No. 402, pp. 1445-1454, July 2004
Journal of Experimental Botany, Vol. 55, No. 402, © Society for Experimental Biology 2004; all rights reserved

REVIEW ARTICLE

Real-time PCR: what relevance to plant studies?

Claire Gachon, Annaïck Mingam and Bénédicte Charrier*

Institut de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris-Sud, F-91405 Orsay cedex, France

* To whom correspondence should be addressed. Fax: +33 1 6915 3425. E-mail: charrier{at}ibp.u-psud.fr

The appearance of genetically modified organisms on the food market a few years ago, and the demand for more precise and reliable techniques to detect foreign (transgenic or pathogenic) DNA in edible plants, have been the driving force for the introduction of real-time PCR techniques in plant research. This was followed by numerous fundamental research applications aiming to study the expression profiles of endogenous genes and multigene families. Since then, the interest in this technique in the plant scientist community has increased exponentially. This review describes the technical features of quantitative real-time PCR that are especially relevant to plant research, and summarizes its present and future applications.

Key words: Expression, fluorochrome, genetically modified organism, Molecular Beacon, pathogen, ScorpionTM, SYBRgreen®, TaqMan®, technique, transgene


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