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JXB Advance Access originally published online on September 24, 2004
Journal of Experimental Botany 2004 55(408):2483-2493; doi:10.1093/jxb/erh254
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Journal of Experimental Botany, Vol. 55, No. 408, © Society for Experimental Biology 2004; all rights reserved

RESEARCH PAPER

A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress

Gisela Mir1, Jordi Domènech2, Gemma Huguet1, Woei-Jiun Guo3, Peter Goldsbrough3, Silvia Atrian2,* and Marisa Molinas1

1Departament de Biologia, Universitat de Girona, Campus Montilivi, E-17071 Girona, Spain
2Departament de Genètica, Universitat de Barcelona, Av. Diagonal 645, E-08028 Barcelona, Spain
3Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA

* To whom correspondence should be addressed. Fax: +34 93 4034420. E-mail: satrian{at}ub.edu

Expression of plant metallothionein genes has been reported in a variety of senescing tissues, such as leaves and stems, ripening fruits, and wounded tissues, and has been proposed to function in both metal chaperoning and scavenging of reactive oxygen species. In this work, it is shown that MT is also associated with suberization, after identifying a gene actively transcribed in Quercus suber cork cells as a novel MT. This cDNA, isolated from a phellem cDNA library, encodes a MT that belongs to type 2 plant MTs (QsMT). Expression of the QsMT cDNA in E. coli grown in media supplemented with Zn, Cd, or Cu has yielded recombinant QsMT. Characterization of the respective metal aggregates agrees well with a copper-related biological role, consistent with the capacity of QsMT to restore copper tolerance to a MT-deficient, copper-sensitive yeast mutant. Furthermore, in situ hybridization results demonstrate that RNA expression of QsMT is mainly observed under conditions related to oxidative stress, either endogenous, as found in cork or in actively proliferating tissues, or exogenous, for example, in response to H2O2 or paraquat treatments. The putative role of QsMT in oxidative stress, both as a free radical scavenger via its sulphydryl groups or as a copper chelator is discussed.

Key words: In situ hybridization, metal co-ordination, metallothionein, oxidative stress, senescent plant tissue, Quercus suber


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