Skip Navigation


JXB Advance Access originally published online on September 10, 2004
Journal of Experimental Botany 2004 55(408):2505-2512; doi:10.1093/jxb/erh256
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/408/2505    most recent
erh256v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Babourina, O.
Right arrow Articles by Newman, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Babourina, O.
Right arrow Articles by Newman, I.
Agricola
Right arrow Articles by Babourina, O.
Right arrow Articles by Newman, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 408, © Society for Experimental Biology 2004; all rights reserved

RESEARCH PAPER

Ion flux interaction with cytoplasmic streaming in branchlets of Chara australis

Olga Babourina*, Konstantin Voltchanskii and Ian Newman

School of Mathematics and Physics, University of Tasmania, GPO Box 252-21, Hobart, Australia 7001

* Present address and to whom correspondence should be sent: School of Earth and Geographical Sciences, M087, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia. Fax: +61 8 9380 1050. E-mail: obabouri{at}agric.uwa.edu.au

Both parts of the actin–myosin complex involved in cytoplasmic streaming could be regulated by mineral ions. The main goal of this study was to find a relationship between cyclosis and ion transport across the cell wall and plasma membrane. The transport of K+ and Ca2+ along pH bands in Chara branchlet internodal cells was characterized by using the MIFE system for non-invasive microelectrode measurement of ion fluxes. Branchlets formed acidic and alkaline bands with the pH ranging from 5 to 8. Different pH patterns were observed for different sides of the branchlets. Sides with cyclosis streaming acropetally generally showed greater variation in the profiles of pH and H+ fluxes. Although a high correlation was not found between pH bands and Ca2+ or K+ fluxes, there was a positive correlation between Ca2+ and K+ fluxes themselves for both sides of the branchlets. Application of cytochalasin D, an inhibitor of cyclosis, had no immediate effect on pH and ion fluxes, however, the time of cyclosis cessation corresponded with a dramatic change in Ca2+ and K+ fluxes; pH profiles and H+ fluxes were affected within 2 h. The evidence suggests that, in Chara branchlets, pH band formation and Gd3+-insensitive Ca2+ transport systems are linked to the cyclosis machinery: (i) the pH band amplitude for the acropetally streaming side was larger than that for the basipetally streaming side; (ii) cessation of cytoplasmic streaming after cytochalasin D application resulted in changed pH banding profiles and H+, Ca2+ and K+ fluxes; and (iii) the application of GdCl3 or incubation in GdCl3 solutions did not lead to the cessation of cytoplasmic streaming, although external Ca2+ fluxes changed.

Key words: Calcium, cyclosis, cytochalasin D, pH, potassium, proton


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
I. Foissner and G. O. Wasteneys
Wide-Ranging Effects of Eight Cytochalasins and Latrunculin A and B on Intracellular Motility and Actin Filament Reorganization in Characean Internodal Cells
Plant Cell Physiol., April 1, 2007; 48(4): 585 - 597.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.