Skip Navigation


JXB Advance Access originally published online on September 24, 2004
Journal of Experimental Botany 2004 55(408):2523-2531; doi:10.1093/jxb/erh266
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/408/2523    most recent
erh266v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Mojovic, M.
Right arrow Articles by Vucinic, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mojovic, M.
Right arrow Articles by Vucinic, Z.
Agricola
Right arrow Articles by Mojovic, M.
Right arrow Articles by Vucinic, Z.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 408, © Society for Experimental Biology 2004; all rights reserved

RESEARCH PAPER

Oxygen radicals produced by plant plasma membranes: an EPR spin-trap study

Milos Mojovic1, Mirjana Vuletic2, Goran G. Bacic1 and Zeljko Vucinic3,*

1Faculty of Physical Chemistry, University of Belgrade, Studentski trg 12–16, YU-11000 Belgrade, Yugoslavia (Serbia and Montenegro)
2Maize Research Institute ‘Zemun Polje’, POB 89–Zemun, YU-11081 Belgrade, Yugoslavia (Serbia and Montenegro)
3Center for Multidisciplinary Studies of the Belgrade University, Ul. Kneza Viseslava 1a, POB 33, YU-11030 Belgrade, Yugoslavia (Serbia and Montenegro)

* To whom correspondence should be addressed. Fax: +381 11 3055 289. E-mail: vucinic{at}ibiss.bg.ac.yu

Plant plasma membranes are known to produce superoxide radicals, while the production of the hydroxyl radical, previously detected in complex plant tissues, is thought to occur in the cell wall. The mechanism of production of superoxide radicals by plant plasma membranes is, however, under dispute. It is shown, using electron paramagnetic resonance spectroscopy with a 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide spin-trap capable of differentiating between radical species, that isolated purified plasma membranes from maize roots produce hydroxyl radicals besides superoxide radicals. The results argue in favour of superoxide production through an oxygen and diphenylene iodonium-sensitive, NADH-dependent superoxide synthase mechanism, as well as through other unidentified mechanism(s). The hydroxyl radical is produced by an oxygen-insensitive, NADH-stimulated mechanism, which is enhanced in membranes in which the superoxide synthase is incapacitated by substrate removal or inhibition.

Key words: DEPMPO, diphenylene iodonium, EPR, hydroxyl, KCN, maize, plasma membranes, spin-trap, superoxide


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
B. Kukavica, M. Mojovic, Z. Vuccinic, V. Maksimovic, U. Takahama, and S. V. Jovanovic
Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production
Plant Cell Physiol., February 1, 2009; 50(2): 304 - 317.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Schopfer, E. Heyno, F. Drepper, and A. Krieger-Liszkay
Naphthoquinone-Dependent Generation of Superoxide Radicals by Quinone Reductase Isolated from the Plasma Membrane of Soybean
Plant Physiology, June 1, 2008; 147(2): 864 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Gapper and L. Dolan
Control of Plant Development by Reactive Oxygen Species
Plant Physiology, June 1, 2006; 141(2): 341 - 345.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.