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JXB Advance Access originally published online on November 22, 2004
Journal of Experimental Botany 2005 56(412):507-513; doi:10.1093/jxb/eri028
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Journal of Experimental Botany, Vol. 56, No. 412, © Society for Experimental Biology 2004; all rights reserved

RESEARCH PAPER

Reduction of cell size induced by enod40 in Arabidopsis thaliana

Flavia Guzzo1, Paolo Portaluppi1, Romeo Grisi1, Silvia Barone1, Stefania Zampieri1, Henk Franssen2 and Marisa Levi1,*

1University of Verona, Dipartimento Scientifico e Tecnologico, Strada le Grazie 15, Cà Vignal 1, 37134 Verona, Italy
2Wageningen University, Department of Molecular Biology, Drijenlaan 3, 6703 HA Wageningen, The Netherlands

* To whom correspondence should be addressed. Fax: +39 45 8027929. E-mail: marisa.levi{at}univr.it

An extensive analysis of organ and cell size was performed in three different Arabidopsis lines transformed with the early nodulin gene enod40 under control of the CaMV35S promoter. All three transgenic lines presented a significant decrease in the mean size of both epidermal internode and leaf mesophyll cells. Flow cytometric and image analysis of enod40-transfected protoplasts prepared from wild-type Arabidopsis cell suspensions showed that transient expression of the gene resulted in reduced forward light scattering (a factor correlated with particle size) and cell size. The direct administration of ENOD40 peptide to fresh protoplasts also resulted in reduced forward scattering with respect to the control and to the administration of unrelated peptides. As far as is known this is the first report documenting a biological effect of enod40 at the cellular level in non-legume plants.

Key words: Arabidopsis, cell size, enod40, flow cytometry, GFP


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