Skip Navigation


JXB Advance Access originally published online on January 10, 2005
Journal of Experimental Botany 2005 56(412):597-603; doi:10.1093/jxb/eri050
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
56/412/597    most recent
eri050v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Bensmihen, S.
Right arrow Articles by Parcy, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bensmihen, S.
Right arrow Articles by Parcy, F.
Agricola
Right arrow Articles by Bensmihen, S.
Right arrow Articles by Parcy, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 56, No. 412, © Society for Experimental Biology 2005; all rights reserved

RESEARCH PAPER

Characterization of three homologous basic leucine zipper transcription factors (bZIP) of the ABI5 family during Arabidopsis thaliana embryo maturation

Sandra Bensmihen*, Jérôme Giraudat and François Parcy {dagger}

Institut des Sciences du Végétal, UPR 2355 CNRS, 1. avenue de la terrasse, 91198 Gif-sur-Yvette cedex, France

* Present address and to whom correspondence should be sent: John Innes Centre, Cell and Developmental Biology Department, Colney Lane, Norwich NR4 7UH, UK. Fax: +44 (0)1603 450 025. E-mail: sandra.bensmihen{at}bbsrc.ac.uk

The Arabidopsis thaliana genome contains approximately 80 genes encoding basic leucine zipper transcription factors, divided into 11 groups. Abscisic Acid-Insensitive 5 (ABI5) is one of the 13 members of group A and is involved in ABA signalling during seed maturation, and germination. Seven other members of this group are expressed during seed maturation, but only one of them (Enhanced Em Level, EEL) has been functionally characterized during this developmental phase. Since EEL and two other group A genes, AtbZIP67 and AREB3 (ABA-Responsive Element Binding protein 3), display similar mRNA temporal expression in whole siliques, it is suspected that they might share some overlapping functions. To address this question, the proteins' tissular and subcellular localization in transgenic Arabidopsis were precisely characterized, using translational fusions with a green fluorescent protein (GFP) expressed under the corresponding bZIP promoter. It was found that the three fusion proteins were expressed with a largely overlapping pattern and constitutively localized in the nuclei. An RNA interference approach (RNAi) was then used to knock out the expression of all three genes simultaneously. Endogenous EEL, AREB3, and AtbZIP67 transcripts could be specifically reduced, but no visible defects could be observed during seed maturation.

Key words: ABI5, bZIP transcription factors, multigene family, RNA interference, seed maturation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
Y. Xiang, N. Tang, H. Du, H. Ye, and L. Xiong
Characterization of OsbZIP23 as a Key Player of the Basic Leucine Zipper Transcription Factor Family for Conferring Abscisic Acid Sensitivity and Salinity and Drought Tolerance in Rice
Plant Physiology, December 1, 2008; 148(4): 1938 - 1952.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
C. D. Deppmann, R. S. Alvania, and E. J. Taparowsky
Cross-Species Annotation of Basic Leucine Zipper Factor Interactions: Insight into the Evolution of Closed Interaction Networks
Mol. Biol. Evol., August 1, 2006; 23(8): 1480 - 1492.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.