Skip Navigation


JXB Advance Access originally published online on December 23, 2004
Journal of Experimental Botany 2005 56(412):713-723; doi:10.1093/jxb/eri038
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/412/713    most recent
eri038v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Tirajoh, A.
Right arrow Articles by Plant, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tirajoh, A.
Right arrow Articles by Plant, A. L.
Agricola
Right arrow Articles by Tirajoh, A.
Right arrow Articles by Plant, A. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 56, No. 412, © Society for Experimental Biology 2004; all rights reserved

RESEARCH PAPER

Stress-responsive {alpha}-dioxygenase expression in tomato roots

Ananchanok Tirajoh, Theingi S. T. Aung, Ashley Byun McKay and Aine L. Plant*

Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada V5A 1S6

* To whom correspondence should be addressed. Fax: +1 604 291 3496. E-mail: aplant{at}sfu.ca

Alpha-dioxygenase ({alpha}-DOX) enzymes catalyse the oxygenation of fatty acids to yield a newly identified group of oxylipins that play a role in protecting tissues from oxidative damage and cell death. In tomato (Lycopersicon esculentum Mill.) {alpha}-DOX was identified as salt-regulated by differential display of mRNA, and is represented by a small gene family comprising at least three members: LE{alpha}-DOX1, -2, and -3 of which only LE{alpha}-DOX1 was salt-responsive. The enhancement of LE{alpha}-DOX1 expression in roots by salt, wounding and challenge with Pythium aphanidermatum (Edson) Fitzp. suggests that {alpha}-DOX-generated oxylipins may mediate the response of roots to these environmental stresses. In roots, LE{alpha}-DOX1 was abscisic acid (ABA)-responsive. However, in the ABA-deficient mutant flacca salt-responsive expression was equivalent to that in the wild type. Similarly, in roots exposed to fluridone (FLU) salt up-regulated expression; however, in this case salt-responsive LE{alpha}-DOX1 expression was greater than that in roots that were not exposed to FLU. A possible explanation for this is provided by the role of ABA in suppressing ethylene accumulation in osmotically stressed roots. The ethylene-generating agent ethephon and precursor 1-aminocyclopropane-1-carboxylic acid markedly elevated LE{alpha}-DOX1 expression, and this enhanced expression was suppressed by ABA. LE{alpha}-DOX1 expression in salt-stressed roots was not markedly affected by AVG indicating that ABA may be responsible for enhanced {alpha}-DOX expression in salt-treated roots.

Key words: Abscisic acid, {alpha}-dioxygenase, ethylene, Lycopersicon esculentum, oxylipin, roots, salt stress


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Koszelak-Rosenblum, A. C. Krol, D. M. Simmons, C. C. Goulah, L. Wroblewski, and M. G. Malkowski
His-311 and Arg-559 Are Key Residues Involved in Fatty Acid Oxygenation in Pathogen-inducible Oxygenase
J. Biol. Chem., September 5, 2008; 283(36): 24962 - 24971.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.