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JXB Advance Access originally published online on January 24, 2005
Journal of Experimental Botany 2005 56(413):887-895; doi:10.1093/jxb/eri081
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

RESEARCH PAPER

Regulation by Vrn-1/Fr-1 chromosomal intervals of CBF-mediated Cor/Lea gene expression and freezing tolerance in common wheat

Fuminori Kobayashi1, Shigeo Takumi1,*, Shinobu Kume1, Machiko Ishibashi1, Ryoko Ohno1, Koji Murai2 and Chiharu Nakamura1

1Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, and Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan
2Department of Bioscience, Fukui Prefectural University, Matsuoka-cho, Fukui, 910-1195, Japan

* To whom correspondence should be addressed. Fax: +81 78 803 5859. E-mail: takumi{at}kobe-u.ac.jp

Vrn-1/Fr-1 chromosomal regions of common wheat possess major QTLs for both winter hardiness (Fr) and vernalization requirement (Vrn). The Vrn-1/Fr-1 intervals are assigned to long arms of the homoeologous group 5 chromosomes. To investigate the role of the Vrn-1/Fr-1 intervals on the low-temperature (LT) inducibility of wheat Cor/Lea genes and its putative transcription factor gene Wcbf2, LT response of these genes was monitored using near-isogenic lines (NILs) for the Vrn-1 loci. The Wcbf2 transcript accumulated rapidly after LT treatment and remained at a high level in lines without any dominant Vrn-1 alleles. By contrast, the Wcbf2 transcript level was greatly reduced in lines carrying the Vrn-1 alleles. The Vrn-1 NILs accumulated much lower amounts of Cor/Lea transcripts and COR/LEA proteins than the non-carrier line. The observed patterns and levels of gene expression, particularly in the Vrn-A1 NIL, agreed with the higher sensitivity to freezing damage in this line than in the non-carrier line. Up-regulation of the expression of the WAP1 gene, a candidate of the Vrn-1 loci, was much delayed in the non-carrier line than all the NILs carrying the Vrn-1 loci. Neither positive nor negative relationships were found between the WAP1 expression and the Cbf2/Cor/Lea expression. These results support the intimate relationship between the Cbf2/Cor/Lea expression and the level of freezing tolerance, and suggest that a functional Fr-A1 allele linked to the vrn-A1 allele, instead of the vernalization gene itself, plays a major role in regulating the CBF-mediated Cor/lea gene expression in wheat.

Key words: Cor/Lea genes, freezing tolerance, homoeologous group 5 chromosomes, Triticum aestivum L, vernalization, WAP1, Wcbf2


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