Skip Navigation


JXB Advance Access originally published online on May 16, 2005
Journal of Experimental Botany 2005 56(417):1797-1804; doi:10.1093/jxb/eri168
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
56/417/1797    most recent
eri168v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ribeiro, D. T.
Right arrow Articles by Van Sluys, M.-A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ribeiro, D. T.
Right arrow Articles by Van Sluys, M.-A.
Agricola
Right arrow Articles by Ribeiro, D. T.
Right arrow Articles by Van Sluys, M.-A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

RESEARCH PAPER

Functional characterization of the thi1 promoter region from Arabidopsis thaliana

Denise Teixeira Ribeiro1, Leonardo Paiva Farias1, Juliana Dantas de Almeida2, Priscila Mayumi Kashiwabara1, Alberto F. C. Ribeiro3, Marcio C. Silva-Filho2, Carlos Frederico Martins Menck4 and Marie-Anne Van Sluys3,*

1Depto. de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, 277, 05508-900 São Paulo, SP, Brazil
2Depto. de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13400-970 Piracicaba, SP, Brazil
3Depto. de Biologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, 277, 05508-900 São Paulo, SP, Brazil
4Depto. de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, 05508-900 São Paulo, SP, Brazil

* To whom correspondence should be addressed. Fax: +55 11 3091 7547. E-mail: mavsluys{at}ib.usp.br

The Arabidopsis thaliana THI1 protein is involved in thiamine biosynthesis and is targeted to both chloroplasts and mitochondria by N-terminal control regions. To investigate thi1 expression, a series of thi1 promoter deletions were fused to the ß-glucuronidase (GUS) reporter gene. Transgenic plants were generated and expression patterns obtained under different environmental conditions. The results show that expression derived from the thi1 promoter is detected early on during development and continues throughout the plant's life cycle. High levels of GUS expression are observed in both shoots and roots during vegetative growth although, in roots, expression is restricted to the vascular system. Deletion analysis of the thi1 promoter region identified a region that is responsive to light. The smallest fragment (designated Pthi322) encompasses 306 bp and possesses all the essential signals for tissue specificity, as well as responsiveness to stress conditions such as sugar deprivation, high salinity, and hypoxia.

Key words: GUS expression, promoter analysis, protein targeting, sugar modulation, thi1, thiamine biosynthesis, tissue expression pattern


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
L. H.M. Ho, E. Giraud, R. Lister, D. Thirkettle-Watts, J. Low, R. Clifton, K. A. Howell, C. Carrie, T. Donald, and J. Whelan
Characterization of the Regulatory and Expression Context of an Alternative Oxidase Gene Provides Insights into Cyanide-Insensitive Respiration during Growth and Development
Plant Physiology, April 1, 2007; 143(4): 1519 - 1533.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.