Skip Navigation


JXB Advance Access originally published online on June 27, 2005
Journal of Experimental Botany 2005 56(418):2019-2028; doi:10.1093/jxb/eri200
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/418/2019    most recent
eri200v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Abirached-Darmency, M.
Right arrow Articles by Thompson, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abirached-Darmency, M.
Right arrow Articles by Thompson, R.
Agricola
Right arrow Articles by Abirached-Darmency, M.
Right arrow Articles by Thompson, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org

RESEARCH PAPER

In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds

M. Abirached-Darmency1,*, M. R. Abdel-gawwad1, G. Conejero2, J. L. Verdeil2 and R. Thompson1

1INRA-URLEG, Unité de Recherche sur les Légumineuses, BP 86510, F-21065 Dijon Cedex, France
2CIRAD UMR BEPC, UMRBPMP TA 40/02 Avenue Agropolis, F-34198, Montpellier cedex 5, France

* To whom correspondence should be addressed. Fax: +33 3 80 69 32 63. E-mail: mona{at}epoisses.inra.fr

The seed consists of several layers of specialized cell-types that divide and differentiate following a highly regulated programme in time and space. A cytological approach was undertaken in order to study the histo-differentiation at mid-embryogenesis in Medicago truncatula as a model legume, and in Pisum sativum using serial sections of embedded immature seed. Little published information is available about seed development in Medicago species. The observations from this study revealed a number of distinctive features of Medicago seed development and differentiation. Transfer cells, involved in nutrient transfer to the embryo, were clearly identified in the thin-walled parenchyma of the innermost integument. Histological Schiff–naphthol enabled carbohydrate accumulation to be followed in the different seed compartments, and revealed the storage protein bodies. Non-radioactive mRNA in situ hybridization, was carried out using mRNA probes from two highly expressed genes encoding the major vicilin and legumin A storage protein types. The timing of mRNA expression was related to that of the corresponding proteins already identified.

Key words: In situ hybridization, legumin A mRNA, Medicago truncatula, mid-embryogenesis, Pisum sativum, seed, vicilin mRNA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.