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JXB Advance Access originally published online on July 4, 2005
Journal of Experimental Botany 2005 56(418):2211-2227; doi:10.1093/jxb/eri221
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org

RESEARCH PAPER

Molecular changes associated with the setting up of secondary growth in aspen

Damien van Raemdonck1, Edouard Pesquet2, Sophie Cloquet1, Hans Beeckman3, Wout Boerjan4, Deborah Goffner2, Mondher El Jaziri1 and Marie Baucher1,*

1Laboratory of Plant Biotechnology, Université Libre de Bruxelles, Chaussée de Wavre 1850, B-1160 Brussels, Belgium
2UMR CNRS/UPS 5546, Surfaces Cellulaires et Signalization chez les Végétaux, Pôle de Biotechnologie Végétale, Chemin de Borde Rouge 24, F-31326 Castanet Tolosan, France
3Laboratory for Wood Biology and Xylarium, Royal Museum for Central Africa, Tervuren, Leuvense Steenweg 13, B-3080 Tervuren, Belgium
4Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Technologiepark 927, B-9052 Gent, Belgium

* To whom correspondence should be addressed. Fax: +32 2 6509175. E-mail: mbaucher{at}ulb.ac.be

Vascular secondary growth results from the activity of the vascular cambium, which produces secondary phloem and secondary xylem. By means of cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis along aspen stems, several potential regulatory genes involved in the progressive transition from primary to secondary growth were identified. A total of 83 unique transcript-derived fragments (TDFs) was found to be differentiated between the top and the bottom of the stem. An independent RT-PCR expression analysis validated the cDNA-AFLP profiles for 19 of the TDFs. Among these, seven correspond to new genes encoding putative regulatory proteins. Emphasis was laid upon two genes encoding, respectively, an AP2/ERF-like transcription factor (PtaERF1) and a RING finger protein (PtaRHE1); their differential expression was further confirmed by reverse northern analysis. In situ RT-PCR revealed that PtaERF1 was expressed in phloem tissue and that PtaRHE1 had a pronounced expression in ray initials and their derivatives within the cambial zone. These results suggest that these genes have a potential role in vascular tissue development and/or functioning.

Key words: AP2/ERF, aspen, cDNA-AFLP, in situ RT-PCR, RING-H2, secondary growth, vascular cambium


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