Skip Navigation


JXB Advance Access originally published online on July 25, 2005
Journal of Experimental Botany 2005 56(419):2433-2442; doi:10.1093/jxb/eri236
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
56/419/2433    most recent
eri236v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Laplaze, L.
Right arrow Articles by Haseloff, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laplaze, L.
Right arrow Articles by Haseloff, J.
Agricola
Right arrow Articles by Laplaze, L.
Right arrow Articles by Haseloff, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

RESEARCH PAPER

GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana

Laurent Laplaze1,2,*, Boris Parizot3, Andrew Baker2, Lilian Ricaud3, Alexandre Martinière1, Florence Auguy1, Claudine Franche1, Laurent Nussaume3, Didier Bogusz1 and Jim Haseloff2

1UMR 1098, Institut de Recherche pour le Développement, 911 Avenue Agropolis, F-34394 Montpellier cedex 5, France
2Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
3Commissariat à l'Energie Atomique Cadarache, DSV, DEVM, Laboratoire de Biologie du Développement des Plantes, 6191 CNRS-CEA, Aix-Marseille II, F-13108 St Paul-lez-Durance Cedex, France

* To whom correspondence should be addressed in France. Fax: +33 4 67 41 62 22. E-mail: laplaze{at}mpl.ird.fr

Lateral root development occurs throughout the life of the plant and is responsible for the plasticity of the root system. In Arabidopsis thaliana, lateral root founder cells originate from pericycle cells adjacent to xylem poles. In order to study the mechanisms of lateral root development, a population of Arabidopsis GAL4-GFP enhancer trap lines were screened and two lines were isolated with GAL4 expression in root xylem-pole pericycle cells (J0121), i.e. in cells competent to become lateral root founder cells, and in young lateral root primordia (J0192). These two enhancer trap lines are very useful tools with which to study the molecular and cellular bases of lateral root development using targeted gene expression. These lines were used for genetic ablation experiments by targeting the expression of a toxin-encoding gene. Moreover, the molecular bases of the enhancer trap expression pattern were characterized. These results suggest that the lateral-root-specific GAL4 expression pattern in J0192 is due to a strong enhancer in the promoter of the LOB-domain protein gene LBD16.

Key words: Genetic ablation, lateral root founder cells, lateral root primordia, patterning, pericycle


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
M. J. Gardner, A. J. Baker, J.-M. Assie, R. S. Poethig, J. P. Haseloff, and A. A. R. Webb
GAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression
J. Exp. Bot., January 1, 2009; 60(1): 213 - 226.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C.-y. Wu, A. Trieu, P. Radhakrishnan, S. F. Kwok, S. Harris, K. Zhang, J. Wang, J. Wan, H. Zhai, S. Takatsuto, et al.
Brassinosteroids Regulate Grain Filling in Rice
PLANT CELL, August 1, 2008; 20(8): 2130 - 2145.
[Abstract] [Full Text] [PDF]

Home page
 CSH ProtocolsHome page
T. D. Southall, D. A. Elliott, and A. H. Brand
The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila
CSH Protocols, July 1, 2008; 2008(8): pdb.top49 - pdb.top49.
[Abstract] [Full Text]

Home page
 Plant CellHome page
E. Truernit, H. Bauby, B. Dubreucq, O. Grandjean, J. Runions, J. Barthelemy, and J.-C. Palauqui
High-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in Arabidopsis
PLANT CELL, June 1, 2008; 20(6): 1494 - 1503.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Derbyshire, S. Drea, P. J. Shaw, J. H. Doonan, and L. Dolan
Proximal-distal patterns of transcription factor gene expression during Arabidopsis root development
J. Exp. Bot., February 7, 2008; (2008) erm301v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Lucas, C. Godin, C. Jay-Allemand, and L. Laplaze
Auxin fluxes in the root apex co-regulate gravitropism and lateral root initiation
J. Exp. Bot., January 1, 2008; 59(1): 55 - 66.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Parizot, L. Laplaze, L. Ricaud, E. Boucheron-Dubuisson, V. Bayle, M. Bonke, I. De Smet, S. R. Poethig, Y. Helariutta, J. Haseloff, et al.
Diarch Symmetry of the Vascular Bundle in Arabidopsis Root Encompasses the Pericycle and Is Reflected in Distich Lateral Root Initiation
Plant Physiology, January 1, 2008; 146(1): 140 - 148.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Laplaze, E. Benkova, I. Casimiro, L. Maes, S. Vanneste, R. Swarup, D. Weijers, V. Calvo, B. Parizot, M. B. Herrera-Rodriguez, et al.
Cytokinins Act Directly on Lateral Root Founder Cells to Inhibit Root Initiation
PLANT CELL, December 1, 2007; 19(12): 3889 - 3900.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. M. Brady, D. A. Orlando, J.-Y. Lee, J. Y. Wang, J. Koch, J. R. Dinneny, D. Mace, U. Ohler, and P. N. Benfey
A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns
Science, November 2, 2007; 318(5851): 801 - 806.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Ohashi-Ito and D. C. Bergmann
Regulation of the Arabidopsis root vascular initial population by LONESOME HIGHWAY
Development, August 15, 2007; 134(16): 2959 - 2968.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Peret, R. Swarup, L. Jansen, G. Devos, F. Auguy, M. Collin, C. Santi, V. Hocher, C. Franche, D. Bogusz, et al.
Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in Casuarina glauca
Plant Physiology, August 1, 2007; 144(4): 1852 - 1862.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
I. De Smet, T. Tetsumura, B. De Rybel, N. F. d. Frey, L. Laplaze, I. Casimiro, R. Swarup, M. Naudts, S. Vanneste, D. Audenaert, et al.
Auxin-dependent regulation of lateral root positioning in the basal meristem of Arabidopsis
Development, February 15, 2007; 134(4): 681 - 690.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
X. Li, X. Mo, H. Shou, and P. Wu
Cytokinin-Mediated Cell Cycling Arrest of Pericycle Founder Cells in Lateral Root Initiation of Arabidopsis
Plant Cell Physiol., August 1, 2006; 47(8): 1112 - 1123.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
A. P. Mahonen, A. Bishopp, M. Higuchi, K. M. Nieminen, K. Kinoshita, K. Tormakangas, Y. Ikeda, A. Oka, T. Kakimoto, and Y. Helariutta
Cytokinin Signaling and Its Inhibitor AHP6 Regulate Cell Fate During Vascular Development
Science, January 6, 2006; 311(5757): 94 - 98.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.