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JXB Advance Access originally published online on August 23, 2005
Journal of Experimental Botany 2005 56(420):2705-2712; doi:10.1093/jxb/eri263
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org

RESEARCH PAPER

Sugar metabolism in developing lupin seeds is affected by a short-term water deficit

Carla Pinheiro1, Ana Paula Rodrigues2, Isabel Saraiva de Carvalho2,3, Maria Manuela Chaves1,2 and Cândido Pinto Ricardo1,2,*

1Instituto de Tecnologia Química e Biológica, Apartado 127, 2781-901 Oeiras, Portugal
2Instituto Superior de Agronomia, Tapada da Ajuda, 1349-017 Lisboa, Portugal
3Faculdade de Engenharia de Recursos Naturais, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal

* To whom correspondence should be addressed. Fax: +351 21 4433644. E-mail: ricardo{at}itqb.unl.pt

A short-term water deficit (WD) imposed during the pre-storage phase of lupin seed development [15–22 d after anthesis (DAA)] accelerated seed maturation and led to smaller and lighter seeds. During seed development, neutral invertase (EC 3.2.1.26) and sucrose synthase (EC 2.4.1.13) have a central role in carbohydrate metabolism. Neutral invertase is predominant during early seed development (up to 40 DAA) and sucrose synthase during the growing and storage phase (40–70 DAA). The contribution of acid invertase is marginal. WD decreased sucrose synthase activity by 2-fold and neutral invertase activity by 5–6-fold. These changes were linked to a large decrease in sucrose (~60%) and an increase of the hexose:sucrose ratio. Rewatering restored sucrose synthase activity to control levels while neutral invertase activity remained depressed (30–60%). A transient accumulation of starch observed in control seeds was abolished by WD. Despite the several metabolic changes the final seed composition was largely unaltered by WD except for ~60% increase in stachyose and raffinose (raffinose family oligosaccharides). This increase in raffinose family oligosaccharides appears as the WD imprinting on mature seeds.

Key words: Galactinol synthase, invertase, Lupinus albus, rewatering, seed development, storage compounds, sucrose synthase, sugars, water deficit


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