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JXB Advance Access originally published online on August 30, 2005
Journal of Experimental Botany 2005 56(420):2721-2731; doi:10.1093/jxb/eri265
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Published by Oxford University Press [2005] on behalf of the Society for Experimental Biology.

RESEARCH PAPER

A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation

Sandrine Mathieu1, Nancy Terrier2,*, Jérôme Procureur1, Frédéric Bigey1 and Ziya Günata1

1UMR IR2B, ENSAM-INRA-Université Montpellier II, F-34060 Montpellier cedex 1, France
2UMR SPO, équipe Biologie Intégrative de la Vigne et du Raisin, ENSAM-INRA, F-34060 Montpellier cedex 1, France

* To whom correspondence should be addressed. Fax: +33 (0)4 99 61 28 57. E-mail: terrier{at}ensam.inra.fr

A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-ß-ionone, a C13-norisoprenoidic compound, and a C14-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C13-norisoprenoid level increased from véraison to maturity in both cultivars.

Key words: 9,10[9',10']-Carotenoid Cleavage Dioxygenase, C13-norisoprenoids, grape berry development, recombinant protein


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