Skip Navigation


JXB Advance Access originally published online on October 10, 2005
Journal of Experimental Botany 2005 56(421):2783-2795; doi:10.1093/jxb/eri271
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/421/2783    most recent
eri271v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (24)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Castro, A. J.
Right arrow Articles by Clément, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Castro, A. J.
Right arrow Articles by Clément, C.
Agricola
Right arrow Articles by Castro, A. J.
Right arrow Articles by Clément, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published by Oxford University Press [2005] on behalf of the Society for Experimental Biology.

RESEARCH PAPER

Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress

Antonio J. Castro1 *, Christine Carapito2, Nathalie Zorn2, Christian Magné1 {dagger}, Emmanuelle Leize2, Alain Van Dorsselaer2 and Christophe Clément1,{ddagger}

1Laboratoire de Stress, Défenses et Reproduction des Plantes, URVVC UPRES EA 2069, Université de Reims Champagne Ardenne, UFR Sciences, BP 1039 Moulin de la Housse, F-51687 Reims cedex 2, France
2Laboratoire de Spectrométrie de Masse Bioorganique, UMR ULP/CNRS 7509, École Européenne de Chimie, Polymères et Matériaux, 25 rue Becquerel, F-67087 Strasbourg cedex 2, France

{ddagger} To whom correspondence should be addressed. Fax: +33 3 26 91 33 39. E-mail: christophe.clement{at}univ-reims.fr

Two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine for the first time the effect of a herbicide (flumioxazin) on a crop species (Vitis vinifera L.) at the proteome level. Examination of 2-D maps derived from chemically stressed tissues revealed the presence of 33 spots displaying a differential expression pattern. The presence of stress responsive proteins in the different plant organs analysed suggests that flumioxazin could act systemically. Among the responsive proteins, some photosynthesis-related proteins, including several fragments of the enzyme Rubisco, were identified. This effect suggests that photosynthesis could be impaired by the herbicide. The induction of several enzymatic antioxidant systems was also observed, probably as a result of an oxidative stress. Moreover, the photorespiration pathway was stimulated, as suggested by the induction of some key enzymes involved in this process. Changes in carbon metabolism-associated proteins presumably reflect altered patterns of carbon flux in response to impaired photosynthesis and an increased need for osmotic adjustment in affected tissues. Finally, plant defences were stimulated as revealed by the induction of a set of proteins belonging to the pathogenesis-related 10 class, suggesting that they could play an essential role in cell defence mechanisms against flumioxazin.

Key words: De novo sequencing, flumioxazin, grapevine, herbicide stress, PRP-10, two-dimensional electrophoresis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 The Plant GenomeHome page
J. Zhu, W. L. Patzoldt, O. Radwan, P. J. Tranel, and S. J. Clough
Effects of Photosystem-II-Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome
The Plant Genome, July 1, 2009; 2(2): 191 - 205.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A.-N. Petit, F. Baillieul, N. Vaillant-Gaveau, L. Jacquens, A. Conreux, P. Jeandet, C. Clement, and F. Fontaine
Low responsiveness of grapevine flowers and berries at fruit set to UV-C irradiation
J. Exp. Bot., March 1, 2009; 60(4): 1155 - 1162.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Zhang, H. Ma, J. Feng, L. Zeng, Z. Wang, and S. Chen
Grape berry plasma membrane proteome analysis and its differential expression during ripening
J. Exp. Bot., August 1, 2008; 59(11): 2979 - 2990.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Enol. Vitic.Home page
M. Troggio, S. Vezzulli, M. Pindo, G. Malacarne, P. Fontana, F. M. Moreira, L. Costantini, M. S. Grando, R. Viola, and R. Velasco
ASEV Honorary Research Lecture 2007: Beyond the Genome, Opportunities for a Modern Viticulture: A Research Overview
Am. J. Enol. Vitic., June 1, 2008; 59(2): 117 - 127.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
Y.-R. Chiang, W. Ismail, D. Heintz, C. Schaeffer, A. Van Dorsselaer, and G. Fuchs
Study of Anoxic and Oxic Cholesterol Metabolism by Sterolibacterium denitrificans
J. Bacteriol., February 1, 2008; 190(3): 905 - 914.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Troggio, G. Malacarne, G. Coppola, C. Segala, D. A. Cartwright, M. Pindo, M. Stefanini, R. Mank, M. Moroldo, M. Morgante, et al.
A Dense Single-Nucleotide Polymorphism-Based Genetic Linkage Map of Grapevine (Vitis vinifera L.) Anchoring Pinot Noir Bacterial Artificial Chromosome Contigs
Genetics, August 1, 2007; 176(4): 2637 - 2650.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Deytieux, L. Geny, D. Lapaillerie, S. Claverol, M. Bonneu, and B. Doneche
Proteome analysis of grape skins during ripening
J. Exp. Bot., May 1, 2007; 58(7): 1851 - 1862.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. Vincent, A. Ergul, M. C. Bohlman, E. A. R. Tattersall, R. L. Tillett, M. D. Wheatley, R. Woolsey, D. R. Quilici, J. Joets, K. Schlauch, et al.
Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity
J. Exp. Bot., May 1, 2007; 58(7): 1873 - 1892.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
U. Roth, E. von Roepenack-Lahaye, and S. Clemens
Proteome changes in Arabidopsis thaliana roots upon exposure to Cd2+
J. Exp. Bot., December 1, 2006; 57(15): 4003 - 4013.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.