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JXB Advance Access originally published online on October 10, 2005
Journal of Experimental Botany 2005 56(421):2783-2795; doi:10.1093/jxb/eri271
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Published by Oxford University Press [2005] on behalf of the Society for Experimental Biology.

RESEARCH PAPER

Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress

Antonio J. Castro1 *, Christine Carapito2, Nathalie Zorn2, Christian Magné1 {dagger}, Emmanuelle Leize2, Alain Van Dorsselaer2 and Christophe Clément1,{ddagger}

1Laboratoire de Stress, Défenses et Reproduction des Plantes, URVVC UPRES EA 2069, Université de Reims Champagne Ardenne, UFR Sciences, BP 1039 Moulin de la Housse, F-51687 Reims cedex 2, France
2Laboratoire de Spectrométrie de Masse Bioorganique, UMR ULP/CNRS 7509, École Européenne de Chimie, Polymères et Matériaux, 25 rue Becquerel, F-67087 Strasbourg cedex 2, France

{ddagger} To whom correspondence should be addressed. Fax: +33 3 26 91 33 39. E-mail: christophe.clement{at}univ-reims.fr

Two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine for the first time the effect of a herbicide (flumioxazin) on a crop species (Vitis vinifera L.) at the proteome level. Examination of 2-D maps derived from chemically stressed tissues revealed the presence of 33 spots displaying a differential expression pattern. The presence of stress responsive proteins in the different plant organs analysed suggests that flumioxazin could act systemically. Among the responsive proteins, some photosynthesis-related proteins, including several fragments of the enzyme Rubisco, were identified. This effect suggests that photosynthesis could be impaired by the herbicide. The induction of several enzymatic antioxidant systems was also observed, probably as a result of an oxidative stress. Moreover, the photorespiration pathway was stimulated, as suggested by the induction of some key enzymes involved in this process. Changes in carbon metabolism-associated proteins presumably reflect altered patterns of carbon flux in response to impaired photosynthesis and an increased need for osmotic adjustment in affected tissues. Finally, plant defences were stimulated as revealed by the induction of a set of proteins belonging to the pathogenesis-related 10 class, suggesting that they could play an essential role in cell defence mechanisms against flumioxazin.

Key words: De novo sequencing, flumioxazin, grapevine, herbicide stress, PRP-10, two-dimensional electrophoresis


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