Skip Navigation


JXB Advance Access originally published online on September 27, 2005
Journal of Experimental Botany 2005 56(421):2907-2914; doi:10.1093/jxb/eri285
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/421/2907    most recent
eri285v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (31)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Nicot, N.
Right arrow Articles by Evers, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nicot, N.
Right arrow Articles by Evers, D.
Agricola
Right arrow Articles by Nicot, N.
Right arrow Articles by Evers, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress

Nathalie Nicot, Jean-François Hausman, Lucien Hoffmann and Danièle Evers*

Centre de Recherche Public-Gabriel Lippmann, Environment and Biotechnologies Research Unit, 41 rue du Brill, L-4422 Belvaux, Luxembourg

* To whom correspondence should be addressed. Fax: +352 47 02 64. E-mail: evers{at}lippmann.lu

Plant stress studies are more and more based on gene expression. The analysis of gene expression requires sensitive, precise, and reproducible measurements for specific mRNA sequences. Real-time RT-PCR is at present the most sensitive method for the detection of low abundance mRNA. To avoid bias, real-time RT-PCR is referred to one or several internal control genes, which should not fluctuate during treatments. Here, the non-regulation of seven housekeeping genes (ß-tubulin, cyclophilin, actin, elongation factor 1-{alpha} (ef1{alpha}), 18S rRNA, adenine phosphoribosyl transferase (aprt), and cytoplasmic ribosomal protein L2) during biotic (late blight) and abiotic stresses (cold and salt stress) was tested on potato plants using geNorm software. Results from the three experimental conditions indicated that ef1{alpha} was the most stable among the seven tested. The expression of the other housekeeping genes tested varied upon stress. In parallel, a study of the variability of expression of hsp20.2, shown to be implicated in late blight stress, was realized. The relative quantification of the hsp20.2 gene varied according to the internal control and the number of internal controls used, thus highlighting the importance of the choice of internal controls in such experiments.

Key words: Abiotic stress, housekeeping genes, late blight, real-time RT-PCR, normalization, Solanum tuberosum


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
M. Kidd, B. Nadler, S. Mane, G. Eick, M. Malfertheiner, M. Champaneria, R. Pfragner, and I. Modlin
GeneChip, geNorm, and gastrointestinal tumors: novel reference genes for real-time PCR
Physiol Genomics, August 20, 2007; 30(3): 363 - 370.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
Z. Zhang and J. Hu
Development and Validation of Endogenous Reference Genes for Expression Profiling of Medaka (Oryzias latipes) Exposed to Endocrine Disrupting Chemicals by Quantitative Real-Time RT-PCR
Toxicol. Sci., February 1, 2007; 95(2): 356 - 368.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.