Skip Navigation


JXB Advance Access originally published online on November 1, 2005
Journal of Experimental Botany 2005 56(422):3207-3214; doi:10.1093/jxb/eri317
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/422/3207    most recent
eri317v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ishimaru, Y.
Right arrow Articles by Nishizawa, N. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishimaru, Y.
Right arrow Articles by Nishizawa, N. K.
Agricola
Right arrow Articles by Ishimaru, Y.
Right arrow Articles by Nishizawa, N. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

OsZIP4, a novel zinc-regulated zinc transporter in rice

Yasuhiro Ishimaru1, Motofumi Suzuki1, Takanori Kobayashi1,2, Michiko Takahashi1, Hiromi Nakanishi3, Satoshi Mori3 and Naoko K. Nishizawa1,2,*

1Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Tokyo, Japan
3Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

* To whom correspondence should be addressed. Fax: +81 3 5841 7514. E-mail: annaoko{at}mail.ecc.u-tokyo.ac.jp

Zinc (Zn) is an essential element for the normal growth of plants but information is scarce on the mechanisms whereby Zn is transported in rice (Oryza sativa L.) plants. Four distinct genes, OsZIP4, OsZIP5, OsZIP6, and OsZIP7 that exhibit sequence similarity to the rice ferrous ion transporter, OsIRT1, were isolated. Microarray and northern blot analysis revealed that OsZIP4 was highly expressed under conditions of Zn deficiency in roots and shoots. Real-time-PCR revealed that the OsZIP4 transcripts were more abundant than those of OsZIP1 or OsZIP3 in Zn-deficient roots and shoots. OsZIP4 complemented a Zn-uptake-deficient yeast (Saccharomyces cerevisiae) mutant, {Delta}zrt1,{Delta}zrt2, indicating that OsZIP4 is a functional transporter of Zn. OsZIP4-synthetic green fluorescent protein (sGFP) fusion protein was transiently expressed in onion epidermal cells localized to the plasma membrane. In situ hybridization analysis revealed that OsZIP4 in Zn-deficient rice was expressed in shoots and roots, especially in phloem cells. Furthermore, OsZIP4 transcripts were detected in the meristem of Zn-deficient roots and shoots. These results suggested that OsZIP4 is a Zn transporter that may be responsible for the translocation of Zn within rice plants.

Key words: IRT1, meristem, phloem, rice, zinc, ZIP4


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. Inoue, T. Kobayashi, T. Nozoye, M. Takahashi, Y. Kakei, K. Suzuki, M. Nakazono, H. Nakanishi, S. Mori, and N. K. Nishizawa
Rice OsYSL15 Is an Iron-regulated Iron(III)-Deoxymugineic Acid Transporter Expressed in the Roots and Is Essential for Iron Uptake in Early Growth of the Seedlings
J. Biol. Chem., February 6, 2009; 284(6): 3470 - 3479.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Cheng, F. Wang, H. Shou, F. Huang, L. Zheng, F. He, J. Li, F.-J. Zhao, D. Ueno, J. F. Ma, et al.
Mutation in Nicotianamine Aminotransferase Stimulated the Fe(II) Acquisition System and Led to Iron Accumulation in Rice
Plant Physiology, December 1, 2007; 145(4): 1647 - 1657.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Ishimaru, H. Masuda, M. Suzuki, K. Bashir, M. Takahashi, H. Nakanishi, S. Mori, and N. K. Nishizawa
Overexpression of the OsZIP4 zinc transporter confers disarrangement of zinc distribution in rice plants
J. Exp. Bot., August 1, 2007; 58(11): 2909 - 2915.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Ishimaru, S. Kim, T. Tsukamoto, H. Oki, T. Kobayashi, S. Watanabe, S. Matsuhashi, M. Takahashi, H. Nakanishi, S. Mori, et al.
From the Cover: Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil
PNAS, May 1, 2007; 104(18): 7373 - 7378.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. Haydon and C. S. Cobbett
A Novel Major Facilitator Superfamily Protein at the Tonoplast Influences Zinc Tolerance and Accumulation in Arabidopsis
Plant Physiology, April 1, 2007; 143(4): 1705 - 1719.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.