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JXB Advance Access originally published online on November 16, 2005
Journal of Experimental Botany 2006 57(1):33-42; doi:10.1093/jxb/erj011
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

FOCUS PAPER

In vivo imaging of MADS-box transcription factor interactions

Isabella A. Nougalli Tonaco1, Jan Willem Borst2,3, Sacco C. de Vries2, Gerco C. Angenent1 and Richard G. H. Immink1,*

1Business Unit Bioscience, Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands
2Department of Biochemistry, Wageningen University, PO Box 8128, 6700 ET Wageningen, The Netherlands
3Microspectroscopy Centre, Wageningen University, PO Box 8128, 6700 ET Wageningen, The Netherlands

* To whom correspondence should be addressed. E-mail: Richard.Immink{at}wur.nl

MADS-box transcription factors are major regulators of development in flowering plants. The factors act in a combinatorial manner, either as homo- or heterodimers, and they control floral organ formation and identity and many other developmental processes through a complex network of protein–protein and protein–DNA interactions. Despite the fact that many studies have been carried out to elucidate MADS-box protein dimerization by yeast systems, very little information is available on the behaviour of these molecules in planta. Here, evidence for specific interactions between the petunia MADS-box proteins FBP2, FBP11, and FBP24 is provided in vivo. The dimers identified in yeast for the ovule-specific FBP24 protein have been confirmed in living plant cells by means of fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy and, in addition, some of the most likely, less stable homo- and heterodimers were identified. This in vivo approach revealed that particular dimers could only be detected in specific sub-nuclear domains. In addition, evidence for the in planta assembly of these ovule-specific MADS-box transcription factors into higher-order complexes is provided.

Key words: FRET-FLIM, in vivo imaging, MADS-box transcription factors


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