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JXB Advance Access originally published online on November 16, 2005
Journal of Experimental Botany 2006 57(1):91-99; doi:10.1093/jxb/erj007
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Effects of genetic manipulation of alcohol dehydrogenase levels on the response to stress and the synthesis of secondary metabolites in grapevine leaves

Catherine Tesniere1,*, Laurent Torregrosa2, Martine Pradal1, Jean-Marc Souquet1, Christine Gilles2, Karine Dos Santos3, Philippe Chatelet2 and Ziya Gunata3

1UMR 1083, Sciences Pour l'Oenologie, Centre INRA/Agro-M, 2 Place Viala, F-34060 Montpellier Cedex 01, France
2UMR 1098, Biologie des Espèces Pérennes Cultivées, Centre INRA/Agro-M, 2 Place Viala, F-34060 Montpellier Cedex 01, France
3UMR1212, Ingénierie des Réactions Biologiques—Bioproductions, Université Montpellier II, Place Eugène Bataillon, F-34095 Montpellier Cedex 05, France

* To whom correspondence should be addressed. Fax: +33 4 99 61 28 57. E-mail: tesniere{at}ensam.inra.fr

The functional role of Adh in regulating susceptibility to abiotic stress and the synthesis of secondary metabolites was investigated in transgenic grapevine plants over- and underexpressing alcohol dehydrogenase (Adh). Plants were transformed with gene constructs containing a sense or antisense orientated grapevine VvAdh2 cDNA under the constitutive cauliflower mosaic virus 35S promoter. Plants transformed with either antisense orientation or the Adh-less construct displayed a low but detectable constitutive ADH activity, whereas plants transformed with the sense-expressed transgene showed a significantly higher (100-fold) ADH activity than the control. Compared with the control, the sense transgene induced an overexpression of VvAdh2 transcripts, whereas a reduced VvAdh2 expression was detected in antisense transformants. Grapevine plants overexpressing Adh displayed a lower sucrose content, a higher degree of polymerization of proanthocyanidins, and a generally increased content of volatile compounds, mainly in carotenoid- and shikimate-derived volatiles. In general, no significant differences between sense/antisense transformants were observed with regard to carotenoid and chlorophyll contents, suggesting a strong metabolic regulation of the synthesis of these compounds.

Key words: Abiotic stress, alcohol dehydrogenase, carotenoids, grapevine, phenolic compounds, sugar content, transformants, volatile compounds


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